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Here we report a serious problem with tobacco gene models. We tried to map the gene models of Nitociana tabacum (TN90), which you provide in your ftp, to the TN90 genome assembly sequences. Unfortunately, a lot of gene models could not be mapped. Of the 122388 gene models you provided in the gene model file,only 53714 could be mapped to the genome assembly. We do not know what method you used to identify gene models. Could you check all the datasets of the three tobacco varieties(TN90, K326 and Bathma)? Thank you.

This topic was started by Peng Wang.
Posted by on 2021-04-29 03:05:06
 
How To Puff Pastry Danish? They tailor-made a foodie tour for me, so I basically was eating all the way, and I really want to share the recipes with you.

When I was in Copenhagen, Denmark, I attended a bakery section. There is a baker instructor, and I spend half a day there.

Together we made five or six kinds of bread, and one of the most famous one is called danish puff pastry, or danish.

Now let’s get started to make one. First, let’s go over the ingredients.

You will need bread flour, milk, butter, egg, honey, salt and instant yeast.

First off, mix all the ingredients (except butter) into a dough. Put them straight in the stand mixer, flour, egg, flour, honey, salt and instant yeast. Do not put salt next to yeast.

Roughly mix it with the dough hook, and then begin to knead the dough.

How To Puff Pastry Danish?
How To Puff Pastry Danish?
How To Puff Pastry Danish?
It takes about five minutes, and it’s the same if you do it manually. Do not knead until glutinous, just stop when the dough is smooth and not sticky.

Touch your dough, if it’s not sticky to touch, take it out and shape it into a ball.

Wrap with a piece of cling film, and chill in the fridge for about half an hour.

Now my butter is softened at room temperature for about 30 minutes. Wrap it in parchment paper, and then beat with a rolling pin.

Do it vertically and horizontally, until it’s flattened.

Once done, carefully fold the parchment paper.

Make it like a rectangular envelope shape.

Now roll out butter.

It’s softened by now, so you can roll it out towards its four corners.

How To Puff Pastry Danish?
How To Puff Pastry Danish?
So that you can roll it out thinly into a rectangular dough sheet.

Feel it with your hand, and even out the surface with rolling pin if not smooth

Now the butter sheet is about 5mm thick. I also measured its size, and it’s 18*23cm.

Now our dough has been sitting for half an hour, transfer it to the floured working surface.

Press it down, and sprinkle some flour.

Roll it out with rolling pin like so.

Roll it into a rectangular dough sheet, and make its width about the same as the length of butter.

That is 23 cm wide. And the length should be 3/2 of the width of butter, and that is 27cm.

Now place butter slice over the dough sheet.

Fold the rest 1/3 dough sheet to the center.

And fold the other end again, and make it a three folds dough.

Stretch the dough if needed, and make sure the butter slice is wrapped inside.

How To Puff Pastry Danish?
How To Puff Pastry Danish?
Roll it out evenly with rolling pin.

Rotate 90 degrees, and try to roll it out into a rectangular dough sheet.

Make another three folds.

Now the dough has been folded twice, and butter is softened. Leave it, wrap with cling film and pop it back to the fridge to chill for another 15 minutes.

The process eases handling of the dough, and also makes the butter solid again.

After 15 minutes, take it out, roll it our evenly with rolling pin, and roll out up and down to make a rectangular dough sheet.

Repeat the folding, and make it three folds.

Wrap with cling film, and chill for 15 minutes again.

Now fold it for the last time. Rotate 90 degrees, roll it out into a rectangular dough sheet.

One last folding. So now the dough has been folded for four times.

Flour a plate, put the dough on it and cover with cling film. Put them back in the fridge to rest for the last time. It takes 30 minutes this time, as long as it’s no longer than 12 hours, it’s fine.

While we are waiting for the dough, prepare a almond paste filling.

You will need softened butter, almond flour and powdered sugar.

First, soften butter at room temperature and beat it with a whisk.

How To Puff Pastry Danish?
How To Puff Pastry Danish?
Add in powdered sugar and mix well.

Switch to a spatula, add in almond flour, and mix well.

Make it like a paste, and this is the filling.

Now the dough is done resting, take it out of the fridge and gently press it down a bit.

And then roll it out evenly with rolling pin

My rolling pin is not long enough, so I have to divide the dough into two parts.

Take one of them on a floured surface, and roll it out thinly into a rectangular dough sheet, about 5-8mm thick.

Cut the dough with a pizza cutter into small portions. I made 8 portions in total.

Scope about a tbs. of almond paste, and then fold the four corners towards the center.

How To Puff Pastry Danish?
How To Puff Pastry Danish?
Pinch the edge together, and this is a classic pasty shape.

Or, you can cut along each diagonal line, but do not cut through.

Scope almond paste in the middle.

Fold the corners to the center following the same direction.

So that you have a pinwheel bread. Or if you have some leftover dough, you can rub almond paste on it, and make a little sandwich.

How To Puff Pastry Danish?
How To Puff Pastry Danish?
Twist it like so , and this is also a type of bread. Now put all the bread dough over a roasting pan lined with parchment paper, cover with cling film and rest aside.

At room temperature 20-25C, rest for about 2 hours until they grow a little bigger.

Now preheat the oven. 180C/256F under fan mode, or 200C/392F for a normal bake mode.

Now press a little dimple in the center.

And brush a thin layer of egg wash.

Scope a spoonful of jam, here I am using marmalade, and for the recipe please click the link.

How To Puff Pastry Danish?
How To Puff Pastry Danish?
You can also substitute with other jams if you like.

Now pop them in the oven, bake for 15 minutes under fan mode.

Until they are golden.

Sprinkle some almond slices on top to garnish. I hope you enjoy this Danish puff pastry.

There are so many fancy ways to make Danish, you can garnish with syrup, almond slices and all kinds of toppings.

For a good puff pastry, if you separate it open, not sure if you can hear the sound or not, but actually there is a the crack sound. And when you bite, it’s crispy on the outside, but very light inside.

How To Puff Pastry Danish8
How To Puff Pastry Danish?
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Posted by on 2021-05-02 21:34:28
 
How To Make Rice Pudding? Today I am making a tutorial on (rice pudding), a dessert that I tried when I was traveling in Turkey. I can never forget its flavor since then.

It is a very common dessert and is found in many places.

Basic ingredients include whole milk, rice, sugar, and corn starch. You also need egg yolk, half vanilla pod, and ground cinnamon to taste.

First off, break the egg.

Separate egg yolk from egg white.

Egg yolk is all you need here.

Add 2 tbs. of milk in the egg yolk.

Mix well into an egg wash.

And then, set aside.

Get 100ml of milk, that is about 6-7 tbs. out of the bowl, and mix with corn starch. And, set aside.

Cut the vanilla pod lengthwise in half.

Use knife tip to scrape out the seeds.

You don’t have to scape out every bit of it. We are going to cook the seeds and the pod together anyway.

Now, place a pot over a stove, and rice in.

Pour in water that is about twice the amount of rice .

You’d better use those short rice here, because it has a higher starch content.

Turn down the heat to low and keep cooking for a few more mins, and make sure all rice absorb enough water.

Cook until you can see no water left at the bottom of the pot, and rice gets soft and tender, and then pour milk into the pot.

Add in vanilla seeds and the pod as well.

How To Make Rice Pudding?
How To Make Rice Pudding?
How To Make Rice Pudding?
Stir constantly while cooking over low heat.

Doing so prevents milk from burning at the bottom, or forming up over the surface.

Keep cooking for about 25 mins or so. Time may various based on what kind of rice you are dealing with.

Just cook until they become soft but not overcooked.

Take out vanilla pod now.

Add in sugar.

And a touch of ground cinnamon.

Stir to dissolve sugar.

Now add in starch water. Starch is denser than water so give it a stir before you add in, so that they won’t go straight down to the bottom.

Stir immediately after you add them in as well and make sure they don’t stick together and become lumpy.

Starch makes the pudding thicker. Now turn off the heat.

Prepare some pudding bowls on a roasting pan.

Divide your pudding mixture in each bowl.

Here I have 6 bowls. Actually it was a mistake.. I ended up fill up only 5 of them.

Next, preheat your oven to 200C/392F.

Now top your puddings with egg wash that we prepared earlier.

How To Make Rice Pudding?
How To Make Rice Pudding?
About 2tsp. in each bowl.

Use the back of a spoon to level off the surface.

Let the egg wash spread evenly.

Set these puddings in the upper level inside your oven.

200C/392F, bake for about 8 mins. The time needed really depends so keep a close attention on it.

It’s ready when it forms a layer of milk at the surface with some caramelized marks.

You can serve it right now.

Or be patient to serve it after cooling, and it will definitely worth the time.

Even though rice pudding is popular in a lot of places, a Turkish rice pudding is different.

On one hand, it has some color on the top with a touch of cinnamon flavor.

It’s not very strong, but it makes the difference.

The texture is very creamy and sweet, with a buttery and tangy fragrant. You can also feel the rice in your mouth.

It’s not too sweet and rich, but very earthy.

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How To Puff Pastry Danish?
Posted by Rick Bertly on 2021-05-28 08:23:38
 
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Posted by Anna Luss on 2021-06-02 08:53:04
 
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Posted by on 2021-06-10 06:48:00
 
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Posted by on 2021-06-15 07:11:47
 
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The second step to getting used to broadcasting is to watch a podcast. A podcast is like a radio program that is broadcast over the Internet using an online interface. A podcast broadcast live on the Internet using a computer, web cam, or video streaming solution. One such online radio station is "DACast" which broadcasts a live video streaming solution.

The third step to getting used to broadcasting is to record your voice and make use of it. People who are familiar with online radio stations have used their recordings to become successful recording artists or have given away their recordings through charity events. It's not difficult to record your own voice and give it away for free as many recording studios now have a "Voices - Share" program that you can use. The recording industry is taking advantage of this new generation of independent artists, who are offering their music for free.
Posted by on 2021-06-15 07:32:22
 
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