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Potato locus granule-bound starch synthase

Locus details

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Locus synonyms 2: GBSS wx [Add/Remove]

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Created on: 2006-12-21 Last updated on: 2011-12-16 by

Potato-TXB 1992

Links to external databases

granule-bound starch synthase is on PhyloGenes

Registry name: granule-bound starch synthase

[Associate registry name]

This locus is associated with registry name: granule-bound starch synthase

Notes and figures (0)

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Accessions and images (0)

[Associate accession]

Alleles (0)

None

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Associated loci (0)

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Associated loci - graphical view

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unprocessed genomic sequence region underlying this gene

>PGSC0003DMG400012112 PGSC_DMv3_219_ch08:41398639..41401252
TTAGAAAGATATTTTTGAGCCCACGACACATTCATTCATAAAGTAAGGTAGTATGTATGATTGAATGGACTACAGCTCAATCAAAGCATCTCCTTTACATAACGGCACCTGTCTCTTTGTCTACTACTCTATTGGTAGTAGTAGTAGTAGTAATTTTACAATCCAAATTGAATAGTAATAAGATGCTCTCTATTTACTAAAGTAGTAGTATTATTCTTTCGTTACTCTAAAGCAACAAATACTATTAAACTTAAAATGCTACTTTTAGGACTAAGTTTTCCCAGTAGCATTTTTTTTTATTTAATAAAGAGAAGATAAAAGCAGGCAAGCCGCTCCGTTACTTATAACACCACCACTCGGCACTCAGCTTACTTTTTTCTAGCTAGGCTGCTGGTTGCTTTTACTCTTTTCTTTCACCTTTAGCTCCTTCCTTCCTTACTTGTCATTTGATGATGGAACCCAATCCCAATCCCAATCCCATCTCCTCTTCTCTTCTCCTCTTTCTGCTGTTTTTTACTCCAACTATGGTGGCTCCTCTAGGTATCAACTACGGGCAAATTGCCAACAATCTTCCCTTACCGGAAAACGTGGTACCATTAGTCAAATCCATCGGAGCTACCAGAATCAAGCTCTATGATGCTGACCCACATGTCCTCAAAGCCTTTGCTAACACCGGCGTTGAGTTCATCGTCAGTCTTGGCAATGAGTACCTTTCCAAGATGAAAGATCCTTCTAAAGCTCAATCATGGGTTAAAAATAATGTCCAAGCTTACTTACCTGCTACTAAAATCACCTGCATCGCTGTCGGTAATGAAGTTCTCACCTTTAACGATACCATCCTCACCGACAACCTCCTCCCTGCAATGCAAAGTGTCCACACTGCTCTTGTTAATCTCAAATTGGACAAACAAGTCACTGTCACCACCGCACATTCTCTTGCTATTCTACAAACTTCCTATCCGCCATCTGCTGGAGCTTTCCGTCGGGATCTTGTCAACTGTGTTACCCAGATCGTGGACTTCCATTGCAAAACTGGCTCACCTTTTCTGATTAATGCTTATCCTTACTTTGCTTACAAGGGAAATCCGAAGCAGGTCTCCCTTGATTTCGTGCTATTTCAGCCCAACTCTGGTATCGTTGATCCAGAGTCCAATCTCCATTACGACAACATGCTTTTCGCTCAGATCGATGCTGTTCACTCTGCTTTAGCTTCCATCGGGTACAAAAACGTCTGCGTACAGATCTCGGAGACGGGATGGCCGTCAAAGGGAGACGCCGACGAGGCCGGAGCTACACCGGAGAATGCTAGGAAATACAACTGTAATCTGATGAAGCTTATTGGTCAGAAGAAGGGTACACCCATGAGGCCTAACTCGGATTTGAATATATACGTATTTGCCTTGTTCAATGAGAACCTCAAGCCTGGTCCTAGTTCGGAGCGAAACTACGGCCTGTTCAAGCCGGACGGATCTCAGGCTTACCCCTTAGGAGTCCCGGCAATCAACGCAGTGAGTACGAATAGCAGCAACTCCGGTGGGTCTGGATCTACACTATCAGCGTCAGTGCCGCCAGCAAGCTCCTCCTCCGGTTATCTAGCCATTACTTCTGATTCGGTGAGCATTTTAGCTTCTTCTTTTTCTCTCTTTTTATTTCCAAGTTCTATAATTACTAAAGGACTTAGATGTATGAAGTTCTGTCTGCTTTCATTTGATACTAATATTCTTGTGCAATTCTCAGGGGACAAGTCTTTCTCCCTGCAAAACCTTGCTTCTCTACTTTGTGAGCTTTGGATTATGGAAGAAACTTGACAACTACTATCACTTGTAATAGACAAGACTCACTCATGTTTGTATTCTTTGATTATTTTTGACGACTCCATTATATTTGCTTTATGGTATTATTATTAGATTAAAAAGACGATACGATAGCTTACCCATTATTCCAATCCGCTCTTATTCACTGGTTTGTTCTAACTTTATGGCTCACTTTCATTCAATTATGTCCATTGTTTTCATCTACAAGAAAATGTCTTAATAAAAACTTCTTCATCAAACTGCTATATGTAATTAGATAAAAAGGTTTGGTCCTTGAGTACAACTTCTTCGCTAAAAAAATGATAAGAACACTACTCTACAAGAGTATAATGCCTCTTACGAATAACTCGGATACCCAAGACTTACAAAATTTCTCTAAAAAAAAAAAAAAGTCTTTCAAGAAACACCAGAGCCTCATCCAATTTCTGTTTTCCTCTTGGTGCCTCTTACTGATAGAGCACCTACTTCTAATTTGCCTAGGATAATATGAGCCCCAACCACGTTAGCTGACTCATGAACTAGCCCCTGCCGGACAGCCAAGAATTCAGACTTTATCAAGAGGGGATAAGAGCAACCAGTCGAGCATCTTCAAGAGCTTCTATGGCACTGAAAGGACCCTCCCACAACTCCATTTTGTTGAGCTTTAACTCTTCGATAGAACAACACATAGGCAGCTGATGTTTTGATATCCGATTCAGCGACAGGTGAAACATGGGAATCATCAAAATGATACCATCTGTCGTCATCAATCAACTGCCAATTAAAAGAACCCATCAGTATACAATGAACCTACAGTCAAC

Download sequence region

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mRNA PGSC0003DMT400031570

Ontology terms

None

terms associated with this mRNA

cDNA sequence

spliced cDNA sequence, including UTRs

>PGSC0003DMT400031570 Glucan endo-1,3-beta-glucosidase
TTAGAAAGATATTTTTGAGCCCACGACACATTCATTCATAAAGTAAGGTAGTATGTATGATTGAATGGACTACAGCTCAATCAAAGCATCTCCTTTACATAACGGCACCTGTCTCTTTGTCTACTACTCTATTGGTAGTAGTAGTAGTAGTAATTTTACAATCCAAATTGAATAGTAATAAGATGCTCTCTATTTACTAAAGTAGTAGTATTATTCTTTCGTTACTCTAAAGCAACAAATACTATTAAACTTAAAATGCTACTTTTAGGACTAAGTTTTCCCAGTAGCATTTTTTTTTATTTAATAAAGAGAAGATAAAAGCAGGCAAGCCGCTCCGTTACTTATAACACCACCACTCGGCACTCAGCTTACTTTTTTCTAGCTAGGCTGCTGGTTGCTTTTACTCTTTTCTTTCACCTTTAGCTCCTTCCTTCCTTACTTGTCATTTGATGATGGAACCCAATCCCAATCCCAATCCCATCTCCTCTTCTCTTCTCCTCTTTCTGCTGTTTTTTACTCCAACTATGGTGGCTCCTCTAGGTATCAACTACGGGCAAATTGCCAACAATCTTCCCTTACCGGAAAACGTGGTACCATTAGTCAAATCCATCGGAGCTACCAGAATCAAGCTCTATGATGCTGACCCACATGTCCTCAAAGCCTTTGCTAACACCGGCGTTGAGTTCATCGTCAGTCTTGGCAATGAGTACCTTTCCAAGATGAAAGATCCTTCTAAAGCTCAATCATGGGTTAAAAATAATGTCCAAGCTTACTTACCTGCTACTAAAATCACCTGCATCGCTGTCGGTAATGAAGTTCTCACCTTTAACGATACCATCCTCACCGACAACCTCCTCCCTGCAATGCAAAGTGTCCACACTGCTCTTGTTAATCTCAAATTGGACAAACAAGTCACTGTCACCACCGCACATTCTCTTGCTATTCTACAAACTTCCTATCCGCCATCTGCTGGAGCTTTCCGTCGGGATCTTGTCAACTGTGTTACCCAGATCGTGGACTTCCATTGCAAAACTGGCTCACCTTTTCTGATTAATGCTTATCCTTACTTTGCTTACAAGGGAAATCCGAAGCAGGTCTCCCTTGATTTCGTGCTATTTCAGCCCAACTCTGGTATCGTTGATCCAGAGTCCAATCTCCATTACGACAACATGCTTTTCGCTCAGATCGATGCTGTTCACTCTGCTTTAGCTTCCATCGGGTACAAAAACGTCTGCGTACAGATCTCGGAGACGGGATGGCCGTCAAAGGGAGACGCCGACGAGGCCGGAGCTACACCGGAGAATGCTAGGAAATACAACTGTAATCTGATGAAGCTTATTGGTCAGAAGAAGGGTACACCCATGAGGCCTAACTCGGATTTGAATATATACGTATTTGCCTTGTTCAATGAGAACCTCAAGCCTGGTCCTAGTTCGGAGCGAAACTACGGCCTGTTCAAGCCGGACGGATCTCAGGCTTACCCCTTAGGAGTCCCGGCAATCAACGCAGTGAGTACGAATAGCAGCAACTCCGGTGGGTCTGGATCTACACTATCAGCGTCAGTGCCGCCAGCAAGCTCCTCCTCCGGTTATCTAGCCATTACTTCTGATTCGGGGACAAGTCTTTCTCCCTGCAAAACCTTGCTTCTCTACTTTGTGAGCTTTGGATTATGGAAGAAACTTGACAACTACTATCACTTGTAATAGACAAGACTCACTCATGTTTGTATTCTTTGATTATTTTTGACGACTCCATTATATTTGCTTTATGGTATTATTATTAGATTAAAAAGACGATACGATAGCTTACCCATTATTCCAATCCGCTCTTATTCACTGGTTTGTTCTAACTTTATGGCTCACTTTCATTCAATTATGTCCATTGTTTTCATCTACAAGAAAATGTCTTAATAAAAACTTCTTCATCAAACTGCTATATGTAATTAGATAAAAAGGTTTGGTCCTTGAGTACAACTTCTTCGCTAAAAAAATGATAAGAACACTACTCTACAAGAGTATAATGCCTCTTACGAATAACTCGGATACCCAAGACTTACAAAATTTCTCTAAAAAAAAAAAAAAGTCTTTCAAGAAACACCAGAGCCTCATCCAATTTCTGTTTTCCTCTTGGTGCCTCTTACTGATAGAGCACCTACTTCTAATTTGCCTAGGATAATATGAGCCCCAACCACGTTAGCTGACTCATGAACTAGCCCCTGCCGGACAGCCAAGAATTCAGACTTTATCAAGAGGGGATAAGAGCAACCAGTCGAGCATCTTCAAGAGCTTCTATGGCACTGAAAGGACCCTCCCACAACTCCATTTTGTTGAGCTTTAACTCTTCGATAGAACAACACATAGGCAGCTGATGTTTTGATATCCGATTCAGCGACAGGTGAAACATGGGAATCATCAAAATGATACCATCTGTCGTCATCAATCAACTGCCAATTAAAAGAACCCATCAGTATACAATGAACCTACAGTCAAC

Protein sequence

translated polypeptide sequence

Gene model matches

SGN Unigenes

[Associate new unigene]

GenBank accessions

[Associate new genbank sequence]

X58453 Potato gene for granule-bound starch synthase.
X52416 S.tuberosum waxy gene for starch synthase (clone G1WxSt).
X66826 S.tuberosum Amf locus, Waxy locus DNA encoding pseudogene similar to beta-1,3-glucanases and beta-1,3-1,4-glucanases.
X83220 S.tuberosum GBSS gene.
AY875539 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875540 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875541 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875542 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875543 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875544 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875545 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875546 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875547 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875548 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875549 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875550 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875551 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875552 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875553 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875554 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY875555 Solanum tuberosum granule-bound starch synthase (waxy) gene, partial sequence.
AY948720 Solanum tuberosum clone NU-YXL-III genomic sequence.
DQ141600 Solanum tuberosum cultivar Atlantic granule-bound starch synthase (GBSS) gene, promoter region and 5' UTR.
DQ387451 Solanum tuberosum granule-bound starch synthase mRNA, partial cds.

Other genome matches

None

Literature annotations [4]

[Associate publication] [Matching publications]

Potato granule-bound starch synthase promoter-controlled GUS expression: regulation of expression after transient and stable transformation. Plant molecular biology (1992)
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Chimaeric genes of promoter sequences from the potato gene encoding granule-bound starch synthase (GBSS) and the beta-glucoronidase (GUS) reporter gene were used to study GBSS expression and regulation. Analysis of stable transformants revealed that a GBSS promoter sequence of 0.4 kb was sufficient to result in tissue-dependent GUS expression: levels in stably transformed microtubers exceeded levels in corresponding leaves by orders of magnitude. GBSS-GUS constructs could be transiently expressed in leaf protoplasts from wild-type and amylose-free potato lines, etuberosum Solanum brevidens, Nicotiana tabacum and Arabidopsis thaliana. Transient expression levels in potato leaf protoplasts were clearly lower than in corresponding suspension cell protoplasts. This lower expression in leaf protoplasts could not be elevated by increasing DNA concentrations during transfection. Light incubation of electroporated suspension cell protoplasts reduced transient GBSS-GUS expression, whereas incubation of transfected protoplasts in media with different sucrose concentrations did not affect transient expression levels. However, electroporated protoplasts, isolated from suspensions, which had been grown on media with increasing amounts of sucrose showed a sucrose concentration-dependent transient expression profile. This indicates that studying GBSS regulation by transient expression experiments needs pre-treatment of the protoplast source. Sequence data of the GBSS promoter were compared to those of two other potato alleles.

van der Steege, G. Nieboer, M. Swaving, J. Tempelaar, MJ. Plant molecular biology. 1992. 20(1). 19-30.

Sequence of the structural gene for granule-bound starch synthase of potato (Solanum tuberosum L.) and evidence for a single point deletion in the amf allele. Molecular & general genetics : MGG (1991)
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The genomic sequence of the potato gene for starch granule-bound starch synthase (GBSS; "waxy protein") has been determined for the wild-type allele of a monoploid genotype from which an amylose-free (amf) mutant was derived, and for the mutant part of the amf allele. Comparison of the wild-type sequence with a cDNA sequence from the literature and a newly isolated cDNA revealed the presence of 13 introns, the first of which is located in the untranslated leader. The promoter contains a G-box-like sequence. The deduced amino acid sequence of the precursor of GBSS shows a high degree of identity with monocot waxy protein sequences in the region corresponding to the mature form of the enzyme. The transit peptide of 77 amino acids, required for routing of the precursor to the plastids, shows much less identity with the transit peptides of the other waxy preproteins, but resembles the hydropathic distributions of these peptides. Alignment of the amino acid sequences of the four mature starch synthases with the Escherichia coli glgA gene product revealed the presence of at least three conserved boxes; there is no homology with previously proposed starch-binding domains of other enzymes involved in starch metabolism. We report the use of chimeric constructs with wild-type and amf sequences to localize, via complementation experiments, the region of the amf allele in which the mutation resides. Direct sequencing of polymerase chain reaction products confirmed that the amf mutation is a deletion of a single AT basepair in the region coding for the transit peptide.(ABSTRACT TRUNCATED AT 250 WORDS)

van der Leij, FR. Visser, RG. Ponstein, AS. Jacobsen, E. Feenstra, WJ. Molecular & general genetics : MGG. 1991. 228(1-2). 240-8.

A putative beta-glucanase pseudogene behind the potato GBSS gene. Plant molecular biology (1993)
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We identified an open reading frame (ORF) which is located closely behind the gene encoding granule-bound starch synthase (GBSS) of potato (Solanum tuberosum L.). The ORF ends with a perfect 43 bp direct repeat, which carries the stop triplet precisely at the beginning of the second repeat. The deduced protein shows homology with all known isoforms of plant beta-1,3-glucanases and beta-1,3-1,4-glucanases. Although the DNA sequence is unique in potato and tomato (Lycopersicon esculentum L.), no expression of the gene was found in these species. Taken together with the unusual codon usage and length of the predicted protein, this sequence could be a pseudogene.

van der Leij, FR. Abeln, EC. Hesseling, Meinders. Feenstra, WJ. Plant molecular biology. 1993. 21(3). 567-71.

Cloning and functional analysis of a cDNA encoding a novel 139 kDa starch synthase from potato (Solanum tuberosum L.). The Plant journal : for cell and molecular biology (1996)
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Three isoforms of starch synthase were shown to be present in soluble potato tuber extracts by activity staining after native gel electrophoresis. An antibody directed against a domain conserved in starch synthases was used to clone a cDNA for one of these isoforms by screening a tuber-specific expression library. A partial cDNA of 2.6 kbp was obtained and used to isolate a full-length cDNA of 4167 bp. The deduced amino acid sequence identifies the protein as a novel type of starch synthase from potato with a molecular mass of 139.2 kDa for the immature enzyme including its transit peptide. This novel isoform was designated SS III. An analysis of the expression pattern of the gene indicates that SS III is equally expressed in tubers of different developmental stages as well as in sink and source leaves. In several independent transgenic potato lines, where the expression of SS III was repressed using the antisense approach, the activity of a specific starch synthase isoform was reduced to non-detectable levels as determined through activity staining after native gel electrophoresis. The reduction of this isoform of starch synthase leads to the synthesis of a structurally modified starch in the transgenic plants: there is a drastic change in granule morphology and an increased level of covalently linked phosphate.

Abel, GJ. Springer, F. Willmitzer, L. Kossmann, J. The Plant journal : for cell and molecular biology. 1996. 10(6). 981-91.

Ontology annotations (7)

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Genomic details

Os06t0133000-01 mRNA feature details

%2Flocus%2F1642%2Fview%2F locus 1642

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Accessions	Seedlots
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