Clone Read SL_EcoRI0017N23_SP6_293721

Sequence Read 
Read Class
BAC ends
Read ID
SL_EcoRI0017N23_SP6_293721
Primer
SP6
ID from Seq. Facility
SL_EcoRI_017_N23-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
558 trimmed for vector and quality, 955 raw
Genbank Accession
CZ945090
Sequence
>SL_EcoRI0017N23_SP6_293721 (raw untrimmed)
AGTCTGCTAAGTCGCAGNTGACTCTAGAGGATCCCGGGNTACCGAGCTCGAATTCAAACCAATAATTGGTTTTCTATAGCATCCTTAAATTGCTTAGACC
TTCGCTTCATCAAGCTCAAGCTCACAAATGCTACCCTTGTCAACGTTTTCTATGAAACACAAAGAGTCCATGTAAACCTCAAATCAAACAAGAATAATGA
GTAGCAACACTGAAGGAGATCCACATACTTTGGAACTTTATGTCAATCTGACTATCAATAAGCTCGTTTCCACCCAAAACTTCACCAAGGCCACCCCATT
TGTGAGAACCAGATGCATGACAAAACGGAAGGCTGTAATAGTTGTATGTTTCCTGTGGGTTATTGTAGGGCCCAACTTTATTTACCCATAAGGTTACTGG
GTCATCTGATTGATACTGTTGGACAAAACATAGAAGTCAGAAATATTTTTGTTGCATAAACGAAGTCCTGGGTAAGAAACAAAACCACAAGCATATTGCA
GTAATACATCCACTTAATTATGCATATTTTGATACTTCACAAAAATTCACAGGAAACAAAAGTGAGAATAAATATTTTCTCCTCCCTAATAGATTTTTTT
TAGTTTCCATATTTTGCCAAAAGTGAACAAAATGTAGATTCAAAAGAAGCAGAACAAACTTGGCATTTTTTATCATTCCAAGCAAAAAACAAAAGGGAAT
AAGGGTTTAACATCCTTAACCAAACCACCATCCAAGCTTGGGTCTCAAACCAATAAAAAAAATACCCCTCATTTTAAAAAACCCCCCTTTTACCCCCTAA
CTGTTCCCATTTAAGTTTTCCTTTAATTGCTTTAAATCTCCATTTTAAATTGGAAAAAAATTCCTTTCCCTTCTTAAAAAAAATTCTATAAAACCCTTCT
ACCCTTCTAAAAAAAGGGGAAACACATTTTATTCCTTTTTCTCAAAACCCTTTAC
>SL_EcoRI0017N23_SP6_293721 (trimmed for quality and cloning vector)
CAAACCAATAATTGGTTTTCTATAGCATCCTTAAATTGCTTAGACCTTCGCTTCATCAAGCTCAAGCTCACAAATGCTACCCTTGTCAACGTTTTCTATG
AAACACAAAGAGTCCATGTAAACCTCAAATCAAACAAGAATAATGAGTAGCAACACTGAAGGAGATCCACATACTTTGGAACTTTATGTCAATCTGACTA
TCAATAAGCTCGTTTCCACCCAAAACTTCACCAAGGCCACCCCATTTGTGAGAACCAGATGCATGACAAAACGGAAGGCTGTAATAGTTGTATGTTTCCT
GTGGGTTATTGTAGGGCCCAACTTTATTTACCCATAAGGTTACTGGGTCATCTGATTGATACTGTTGGACAAAACATAGAAGTCAGAAATATTTTTGTTG
CATAAACGAAGTCCTGGGTAAGAAACAAAACCACAAGCATATTGCAGTAATACATCCACTTAATTATGCATATTTTGATACTTCACAAAAATTCACAGGA
AACAAAAGTGAGAATAAATATTTTCTCCTCCCTAATAGATTTTTTTTAGTTTCCATAT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
3' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.948175
Expected error %
1.26662
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g10950.1 Score 87 E Value 5e-21 Identity 66.10% Align. Length 321bp (57.53%) Frame 2
68414.m01257 endomembrane protein 70, putative
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_466169.1| Score 91 E Value 3e-19 Identity 69.40% Align. Length 318bp (56.99%) Frame 2
putative transmembrane protein TM9SF3 (66.6 kD) [Oryza sativa (japonica cultivar-group)] ref|XP_506821.1| PREDICTED OJ1004_H01.22 gene product [Oryza sativa (japonica cultivar-group)] dbj|BAD15485.1| putative transmembrane protein TM9SF3 (66.6 kD) [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAP40425.1| Score 87 E Value 2e-19 Identity 66.10% Align. Length 321bp (57.53%) Frame 2
putative endomembrane protein 70 [Arabidopsis thaliana] gb|AAL36263.1| putative endomembrane protein EMP70 precusor isolog [Arabidopsis thaliana] gb|AAM10098.1| endomembrane protein EMP70 precusor isolog [Arabidopsis thaliana] gb|AAL48237.1| At1g10950/T19D16_13 [Arabidopsis thaliana] ref|NP_563881.1| endomembrane protein 70, putative [Arabidopsis thaliana] gb|AAK96857.1| endomembrane protein EMP70 precusor isolog [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone SL_EcoRI0017N23
Clone
Clone name
sle-17n23
SL_EcoRI0017N23
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato EcoRI BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pIndigoBAC-5
Accession
Heinz 1706
Restriction enzyme
EcoRI

Other Reads:
1 sequencing reads found