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SC Spread ID	Images			Distance from centromere (percentage of arm length)
350-04 1	Composite	Fluorescence	Phase	25.1%
350-13 1	Composite	Fluorescence	Phase	28.0%
350-18 1	Composite	Fluorescence	Phase	26.2%
350-26 1	Composite	Fluorescence	Phase	26.6%
350-28 1	Composite	Fluorescence	Phase	24.7%
350-29 1	Composite	Fluorescence	Phase	27.6%
350-30 1	Composite	Fluorescence	Phase	24.5%
350-35 1	Composite	Fluorescence	Phase	28.4%
350-39 1	Composite	Fluorescence	Phase	29.5%
350-40 1	Composite	Fluorescence	Phase	27.9%
350-43 1	Composite	Fluorescence	Phase	26.0%
350-47 1	Composite	Fluorescence	Phase	24.6%
350-48 1	Composite	Fluorescence	Phase	24.7%
350-49 1	Composite	Fluorescence	Phase	26.6%
Mean±sd				26.4%±1.6%

Given an estimated length of 15.7µm for chromosome 3Q and the mean percentage above, this BAC is approximately 4.2µm±0.3µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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