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SC Spread ID	Images			Distance from centromere (percentage of arm length)
586-12 1	Composite	Fluorescence	Phase	28.3%
586-19 1	Composite	Fluorescence	Phase	27.6%
586-31 1	Composite	Fluorescence	Phase	26.6%
586-37 1	Composite	Fluorescence	Phase	28.7%
586-38 1	Composite	Fluorescence	Phase	27.3%
586-41 1	Composite	Fluorescence	Phase	28.4%
586-42 1	Composite	Fluorescence	Phase	28.2%
586-51 1	Composite	Fluorescence	Phase	25.7%
586-52 1	Composite	Fluorescence	Phase	27.1%
586-54 1	Composite	Fluorescence	Phase	28.9%
586-58 1	Composite	Fluorescence	Phase	26.5%
586-60 1	Composite	Fluorescence	Phase	28.8%
586-67 1	Composite	Fluorescence	Phase	26.3%
Mean±sd				27.6%±1.1%

Given an estimated length of 11.7µm for chromosome 8Q and the mean percentage above, this BAC is approximately 3.2µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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