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SC Spread ID	Images			Distance from centromere (percentage of arm length)
523-15 1	Composite	Fluorescence	Phase	23.0%
523-17 1	Composite	Fluorescence	Phase	25.7%
523-18 1	Composite	Fluorescence	Phase	28.3%
523-22 1	Composite	Fluorescence	Phase	25.8%
523-25 1	Composite	Fluorescence	Phase	24.0%
523-26 1	Composite	Fluorescence	Phase	24.3%
523-27 1	Composite	Fluorescence	Phase	28.9%
523-29 1	Composite	Fluorescence	Phase	23.8%
523-30 1	Composite	Fluorescence	Phase	26.4%
523-34 1	Composite	Fluorescence	Phase	27.5%
523-40 1	Composite	Fluorescence	Phase	23.7%
523-44 1	Composite	Fluorescence	Phase	26.4%
523-70 1	Composite	Fluorescence	Phase	26.5%
Mean±sd				25.7%±1.9%

Given an estimated length of 20.1µm for chromosome 1Q and the mean percentage above, this BAC is approximately 5.2µm±0.4µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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