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SC Spread ID	Images			Distance from centromere (percentage of arm length)
160-02 1	Composite	Fluorescence	Phase	37.6%
160-05 1	Composite	Fluorescence	Phase	38.1%
160-07 1	Composite	Fluorescence	Phase	38.2%
160-08 1	Composite	Fluorescence	Phase	35.0%
160-09 1	Composite	Fluorescence	Phase	38.1%
160-19 1	Composite	Fluorescence	Phase	41.4%
160-27 1	Composite	Fluorescence	Phase	38.0%
160-31 1	Composite	Fluorescence	Phase	37.7%
Mean±sd				38.0%±1.7%

Given an estimated length of 13.2µm for chromosome 4Q and the mean percentage above, this BAC is approximately 5.0µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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