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SC Spread ID	Images			Distance from centromere (percentage of arm length)
664-05 1	Composite	Fluorescence	Phase	84.0%
664-06 1	Composite	Fluorescence	Phase	79.3%
664-07 1	Composite	Fluorescence	Phase	82.6%
664-29 1	Composite	Fluorescence	Phase	81.7%
664-30 1	Composite	Fluorescence	Phase	81.7%
664-35 1	Composite	Fluorescence	Phase	79.1%
664-37 1	Composite	Fluorescence	Phase	86.8%
664-38 1	Composite	Fluorescence	Phase	83.2%
664-58 1	Composite	Fluorescence	Phase	81.4%
664-61 1	Composite	Fluorescence	Phase	86.0%
664-62 1	Composite	Fluorescence	Phase	81.7%
664-74 1	Composite	Fluorescence	Phase	84.1%
664-75 1	Composite	Fluorescence	Phase	83.0%
Mean±sd				82.7%±2.3%

Given an estimated length of 6.8µm for chromosome 12P and the mean percentage above, this BAC is approximately 5.6µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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