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SC Spread ID	Images			Distance from centromere (percentage of arm length)
649-07 1	Composite	Fluorescence	Phase	79.0%
649-10 1	Composite	Fluorescence	Phase	76.8%
649-16 1	Composite	Fluorescence	Phase	77.1%
649-19 1	Composite	Fluorescence	Phase	77.6%
649-22 1	Composite	Fluorescence	Phase	81.4%
649-24a 1	Composite	Fluorescence	Phase	76.7%
649-24b 1	Composite	Fluorescence	Phase	77.4%
649-26 1	Composite	Fluorescence	Phase	82.3%
649-28 1	Composite	Fluorescence	Phase	77.4%
649-33 1	Composite	Fluorescence	Phase	78.7%
649-35 1	Composite	Fluorescence	Phase	79.0%
649-40 1	Composite	Fluorescence	Phase	76.2%
649-51 1	Composite	Fluorescence	Phase	82.5%
649-52 1	Composite	Fluorescence	Phase	77.4%
649-54 1	Composite	Fluorescence	Phase	81.3%
649-59 1	Composite	Fluorescence	Phase	81.3%
Mean±sd				78.9%±2.2%

Given an estimated length of 6.8µm for chromosome 12P and the mean percentage above, this BAC is approximately 5.4µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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