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SC Spread ID	Images			Distance from centromere (percentage of arm length)
175-03a 1	Composite	Fluorescence	Phase	22.2%
175-03b 1	Composite	Fluorescence	Phase	26.2%
175-03c 1	Composite	Fluorescence	Phase	23.4%
175-04 1	Composite	Fluorescence	Phase	22.8%
175-06 1	Composite	Fluorescence	Phase	26.6%
175-08 1	Composite	Fluorescence	Phase	27.8%
175-11 1	Composite	Fluorescence	Phase	26.0%
175-17 1	Composite	Fluorescence	Phase	24.2%
175-19 1	Composite	Fluorescence	Phase	25.5%
175-20 1	Composite	Fluorescence	Phase	23.2%
175-21 1	Composite	Fluorescence	Phase	22.6%
175-25 1	Composite	Fluorescence	Phase	23.3%
Mean±sd				24.5%±1.8%

Given an estimated length of 13.2µm for chromosome 4Q and the mean percentage above, this BAC is approximately 3.2µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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