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SC Spread ID	Images			Distance from centromere (percentage of arm length)
205-01 1	Composite	Fluorescence	Phase	4.2%
205-03 1	Composite	Fluorescence	Phase	3.2%
205-04 1	Composite	Fluorescence	Phase	3.7%
205-06 1	Composite	Fluorescence	Phase	4.3%
205-14 1	Composite	Fluorescence	Phase	4.0%
205-16 1	Composite	Fluorescence	Phase	2.7%
205-17 1	Composite	Fluorescence	Phase	2.6%
205-21 1	Composite	Fluorescence	Phase	2.8%
205-22 1	Composite	Fluorescence	Phase	2.3%
205-27 1	Composite	Fluorescence	Phase	3.3%
205-29 1	Composite	Fluorescence	Phase	2.2%
Mean±sd				3.2%±0.8%

Given an estimated length of 18.9µm for chromosome 2Q and the mean percentage above, this BAC is approximately 0.6µm±0.1µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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