Sol Genomics Network

SGN Marker cLEC-7-B23

SGN-M3067

Synonyms

None

Locus associations

None

Derivations

This marker was derived from EST clone SGN-C14174

Mapped locations

Map: Tomato-EXPEN 2000
Map version 52 (location: 40284)
Chromosome 4
Position 101.00 cM
Confidence CF(LOD3)
Protocol CAPS

Tomato-EXPEN 2000

Other PCR data

PCR data Exp. ID 35661
Forward primer (5'-3') GGAGAACACGGCTACCTCAG	Digested band sizes (using CfoI) Solanum pennellii LA716: 600 Solanum lycopersicum LA925: 520	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGCTGGAAATGAGGTTTTGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 600 Solanum pennellii LA716: 600	Approximate temperature 55°C

PCR data Exp. ID 65
Forward primer (5'-3') Unknown	Digested band sizes (using CfoI) Solanum pennellii LA716: 600 Solanum lycopersicum LA925: 520	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') Unknown	Enzymes
Accessions and product sizes Solanum pennellii LA716: 600 Solanum lycopersicum LA925: 600	Approximate temperature 55°C

Overgo hybridization and physical mapping

cLEC-7-B23 was used as an overgo probe on plate 6 [well D12]

Plausible BAC Matches: None

Overgo Sequences

A sequence

GGATGCAGCGTAACATTGCTAACC

B sequence

CCACACAAACCCTTTGGGTTAGCA

AB sequence

GGATGCAGCGTAACATTGCTAACCCAAAGG

Marker sequence

CATGGATTATTGGATTGTGAGGAACTCATGGGGTGCTTCCTGGGGAGAAC
ACGGCTACCTCAGGATGCAGCGTAACATTGCTAACCCAAAGGGTTTGTGT
GGTATTGCTACAGTTGTTTCTTACCCTGTCAAGACAGGCCAAAATCCCCC
GAAACCAGCTCCATCTCCTCCATCGCCAGTCAAGCCACCTACTACTTGTG
ATGATATGTACAGTTGCCCATCAGGAACAACGTGTTGCTGTGTCTACGAG
TACTATCACATGTGCTTTGCTTGGGGTTGCTGCCCTATGGAAGGAGCTAC
TTGCTGTAAAGACCATAACAGTTGCTGCCCACATGATTATCCTGTCTGCA
ATGTTAAAGCCGGCACCTGCTCAATCAGCGAGAATAACCCTCTTTCAGTC
AAAGCAATGTCACACATTCTTGCCAAACCTATTGGATCCTTCAGCAATCA
NGGAATGAAAAACACTATCTCTTGAAGTGTCTCCAATGGGGATGCGCAAA
TTCGCAGTGAATTTGCAAAACCTCATTTCCAGCTTGAAAGTTATAGTTAT

Genomic location of cLEC-7-B23

%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D3067 marker 3067