Sol Genomics Network

SGN-M4719
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	308409 (SGN) 131127 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U204638	-	-	-
Solanum tuberosum	Single	SGN-U246039	-	-	-
Arabidopsis	Single	At2g38025 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39624

Forward primer (5'-3')

ATGGGCGCTGCATGTTTCGTG

Accessions and product sizes

Solanum lycopersicum TA209

1150

Solanum pennellii TA56

1150

Approximate temperature

55°C

Reverse primer (5'-3')

ACACCTTTGTTGAAAGCCATCCC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

500+600

Solanum pennellii TA56

900

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10818
Forward primer (5'-3') ATGGGCGCTGCATGTTTCGTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACACCTTTGTTGAAAGCCATCCC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1150	Approximate temperature 55°C

PCR data Exp. ID 10819
Forward primer (5'-3') ATGGGCGCTGCATGTTTCGTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACACCTTTGTTGAAAGCCATCCC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1150	Approximate temperature 55°C

PCR data Exp. ID 10820
Forward primer (5'-3') ATGGGCGCTGCATGTTTCGTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACACCTTTGTTGAAAGCCATCCC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1150	Approximate temperature 55°C

PCR data Exp. ID 13051
Forward primer (5'-3') ATGGGCGCTGCATGTTTCGTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACACCTTTGTTGAAAGCCATCCC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 1150	Approximate temperature 55°C

PCR data Exp. ID 13052
Forward primer (5'-3') ATGGGCGCTGCATGTTTCGTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACACCTTTGTTGAAAGCCATCCC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1150	Approximate temperature 55°C

PCR data Exp. ID 13053
Forward primer (5'-3') ATGGGCGCTGCATGTTTCGTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACACCTTTGTTGAAAGCCATCCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1150	Approximate temperature 55°C

PCR data Exp. ID 13054
Forward primer (5'-3') ATGGGCGCTGCATGTTTCGTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACACCTTTGTTGAAAGCCATCCC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1150	Approximate temperature 55°C

PCR data Exp. ID 13055
Forward primer (5'-3') ATGGGCGCTGCATGTTTCGTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACACCTTTGTTGAAAGCCATCCC	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 1150	Approximate temperature 55°C

PCR data Exp. ID 32954
Forward primer (5'-3') ATGGGCGCTGCATGTTTCGTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACACCTTTGTTGAAAGCCATCCC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1150	Approximate temperature 55°C

PCR data Exp. ID 32955
Forward primer (5'-3') ATGGGCGCTGCATGTTTCGTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACACCTTTGTTGAAAGCCATCCC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 1150	Approximate temperature 55°C

PCR data Exp. ID 32956
Forward primer (5'-3') ATGGGCGCTGCATGTTTCGTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACACCTTTGTTGAAAGCCATCCC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1150	Approximate temperature 55°C

PCR data Exp. ID 10821
Forward primer (5'-3') ATGGGCGCTGCATGTTTCGTG	Digested band sizes (using DraI) Solanum lycopersicum LA925: 600+500 Solanum pennellii LA716: 900	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACACCTTTGTTGAAAGCCATCCC	Enzymes All possible enzymes for pennellii and and lycopersicum: DraI, DpnII
Accessions and product sizes Solanum lycopersicum LA925: 1150 Solanum pennellii LA716: 1150	Approximate temperature 55°C