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SGN Marker C2_At1g20575

SGN-M6670
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Capsicum annuum	Single	SGN-U197163	-	-	-
Lycopersicon Combined	Single	SGN-U224892	-	-	-
Solanum tuberosum	Single	SGN-U251460	-	-	-
Arabidopsis	Single	At1g20575 on TAIR	-	-	-

Mapped locations

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46564)
Chromosome 4
Position 89.50 cM
Confidence CF(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Map: Tobacco N. acuminata
Map version 89 (location: 46431)
Chromosome 4
Position 74.80 cM
Confidence F(LOD3)
Protocol dCAPS

Tobacco N. acuminata

Map: Pepper-COSII
Map version 58 (location: 41311)
Chromosome 4
Position 145.00 cM
Confidence F(LOD3)
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43287

Forward primer (5'-3')

AGACACTACTTTGGCCGGGTGTATC

Accessions and product sizes

Approximate temperature

53°C

Reverse primer (5'-3')

ATGATGTCTTCTAGTGCAGACTTCTGG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38125)
Chromosome 4
Position 132.70 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39413

Forward primer (5'-3')

AGACACTACTTTGGCCGGGTGTATC

Accessions and product sizes

Solanum pennellii TA56

100

Solanum lycopersicum TA209

100

Approximate temperature

55°C

Reverse primer (5'-3')

ATGATGTCTTCTAGTGCAGACTTCTGG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25074)
Chromosome 1
Position 7.13 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10067
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 600	Approximate temperature 55°C

PCR data Exp. ID 10068
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 600	Approximate temperature 55°C

PCR data Exp. ID 10069
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 600	Approximate temperature 55°C

PCR data Exp. ID 12489
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 500	Approximate temperature 55°C

PCR data Exp. ID 12490
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Solanum melongena TA2986: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12491
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 500	Approximate temperature 55°C

PCR data Exp. ID 12492
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 500	Approximate temperature 55°C

PCR data Exp. ID 12493
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 500	Approximate temperature 55°C

PCR data Exp. ID 12494
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 550	Approximate temperature 55°C

PCR data Exp. ID 12495
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 550	Approximate temperature 55°C

PCR data Exp. ID 12496
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Physalis TA1367: 300	Approximate temperature 55°C

PCR data Exp. ID 12497
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 850	Approximate temperature 55°C

PCR data Exp. ID 12498
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 800	Approximate temperature 55°C

PCR data Exp. ID 12499
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 800	Approximate temperature 55°C

PCR data Exp. ID 12500
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 850	Approximate temperature 55°C

PCR data Exp. ID 32720
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 500	Approximate temperature 55°C

PCR data Exp. ID 32721
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 500	Approximate temperature 55°C

PCR data Exp. ID 32722
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 550	Approximate temperature 55°C

PCR data Exp. ID 32723
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 550	Approximate temperature 55°C

PCR data Exp. ID 10070
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC	Digested band sizes (using TaqI) Solanum lycopersicum LA925: 420 Solanum pennellii LA716: 500	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes All possible enzymes for pennellii and and lycopersicum: MspI, TaqI, HaeIII, HinfI
Accessions and product sizes Solanum pennellii LA716: 600 Solanum lycopersicum LA925: 600	Approximate temperature 55°C

Unigene blast matches for primers

SGN-U197163
SGN-U224892

Universal primers for Asterid species

No additional PCR data found.

Other COSII sequence data

BLASTX result of original unigene sequences against Arabidopsis protein database

BLASTX result of original unigene sequences against Arabidopsis protein database

Related Markers

Related Genotyped Markers: These markers have been genotyped and share a name with this marker

Genomic location of C2_At1g20575

%2Fsearch%2Fmarkers%2Fmarkerinfo.pl%3Fmarker_id%3D6670 marker 6670

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Accessions	Seedlots
Accessions		Breeding Program	Seedlot Name	Contents	Seedlot Location	Count	Weight(g)

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