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SGN Marker C2_At1g20575

SGN-M6670
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Capsicum annuum	Single	SGN-U197163	-	-	-
Lycopersicon Combined	Single	SGN-U224892	-	-	-
Solanum tuberosum	Single	SGN-U251460	-	-	-
Arabidopsis	Single	At1g20575 on TAIR	-	-	-

Mapped locations

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46564)
Chromosome 4
Position 89.50 cM
Confidence CF(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Map: Tobacco N. acuminata
Map version 89 (location: 46431)
Chromosome 4
Position 74.80 cM
Confidence F(LOD3)
Protocol dCAPS

Tobacco N. acuminata

Map: Pepper-COSII
Map version 58 (location: 41311)
Chromosome 4
Position 145.00 cM
Confidence F(LOD3)
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43287

Forward primer (5'-3')

AGACACTACTTTGGCCGGGTGTATC

Accessions and product sizes

Approximate temperature

53°C

Reverse primer (5'-3')

ATGATGTCTTCTAGTGCAGACTTCTGG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38125)
Chromosome 4
Position 132.70 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39413

Forward primer (5'-3')

AGACACTACTTTGGCCGGGTGTATC

Accessions and product sizes

Solanum pennellii TA56

100

Solanum lycopersicum TA209

100

Approximate temperature

55°C

Reverse primer (5'-3')

ATGATGTCTTCTAGTGCAGACTTCTGG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25074)
Chromosome 1
Position 7.13 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10067
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 600	Approximate temperature 55°C

PCR data Exp. ID 10068
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 600	Approximate temperature 55°C

PCR data Exp. ID 10069
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 600	Approximate temperature 55°C

PCR data Exp. ID 12489
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 500	Approximate temperature 55°C

PCR data Exp. ID 12490
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Solanum melongena TA2986: Multiple	Approximate temperature 55°C

PCR data Exp. ID 12491
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 500	Approximate temperature 55°C

PCR data Exp. ID 12492
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 500	Approximate temperature 55°C

PCR data Exp. ID 12493
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 500	Approximate temperature 55°C

PCR data Exp. ID 12494
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 550	Approximate temperature 55°C

PCR data Exp. ID 12495
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 550	Approximate temperature 55°C

PCR data Exp. ID 12496
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Physalis TA1367: 300	Approximate temperature 55°C

PCR data Exp. ID 12497
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Petunia axillaris TA3309: 850	Approximate temperature 55°C

PCR data Exp. ID 12498
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 800	Approximate temperature 55°C

PCR data Exp. ID 12499
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 800	Approximate temperature 55°C

PCR data Exp. ID 12500
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Petunia integrifolia TA3312: 850	Approximate temperature 55°C

PCR data Exp. ID 32720
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 500	Approximate temperature 55°C

PCR data Exp. ID 32721
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 500	Approximate temperature 55°C

PCR data Exp. ID 32722
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 550	Approximate temperature 55°C

PCR data Exp. ID 32723
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 550	Approximate temperature 55°C

PCR data Exp. ID 10070
Forward primer (5'-3') AGACACTACTTTGGCCGGGTGTATC	Digested band sizes (using TaqI) Solanum lycopersicum LA925: 420 Solanum pennellii LA716: 500	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGATGTCTTCTAGTGCAGACTTCTGG	Enzymes All possible enzymes for pennellii and and lycopersicum: MspI, TaqI, HaeIII, HinfI
Accessions and product sizes Solanum lycopersicum LA925: 600 Solanum pennellii LA716: 600	Approximate temperature 55°C