Sol Genomics Network

SGN-M7191
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	306492 (SGN) 126099 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U200690	-	-	-
Lycopersicon Combined	Single	SGN-U222923	-	-	-
Arabidopsis	Single	At2g13540 on TAIR	-	-	-

Arabidopsis COSII

PCR data Exp. ID 41202

Forward primer (5'-3')

TGTTGTTCAAACACTTCTCGACATCGG

Accessions and product sizes

Solanum lycopersicum TA209

900

Solanum pennellii TA56

900

Approximate temperature

55°C

Reverse primer (5'-3')

TCTTACAGGGTTTTCACCAAGAACAGG

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

850

Solanum lycopersicum TA209

550

Mg⁺² concentration

1.5mM

Enzymes

Other PCR data

PCR data Exp. ID 10552
Forward primer (5'-3') TGTTGTTCAAACACTTCTCGACATCGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTTACAGGGTTTTCACCAAGAACAGG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1650	Approximate temperature 55°C

PCR data Exp. ID 10553
Forward primer (5'-3') TGTTGTTCAAACACTTCTCGACATCGG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTTACAGGGTTTTCACCAAGAACAGG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1650	Approximate temperature 55°C

PCR data Exp. ID 10554
Forward primer (5'-3') TGTTGTTCAAACACTTCTCGACATCGG	Digested band sizes (using CfoI) Solanum pennellii LA716: 1650 Solanum lycopersicum LA925: 700+950	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TCTTACAGGGTTTTCACCAAGAACAGG	Enzymes All possible enzymes for pennellii and and lycopersicum: BsiHKAI,CfoI/HhaI,Hpy188I,RsaI,ScaI,TspRI All possible enzymes for lycopersicum and pimpinellifolium: BstBI,TaqI
Accessions and product sizes Solanum lycopersicum LA925: 1650 Solanum pennellii LA716: 1650	Approximate temperature 55°C