Sol Genomics Network

SGN-M7291
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	300660 (SGN) 125168 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U216910	-	-	-
Arabidopsis	Single	At2g24940 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39584

Forward primer (5'-3')

AGCTAACCGCGGATGAGCTGAAG

Accessions and product sizes

Solanum lycopersicum TA209

200

Solanum pennellii TA56

200

Approximate temperature

55°C

Reverse primer (5'-3')

TTGAGAACAGCCATTTCTTTCTCAG

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

170

Solanum pennellii TA56

200

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10656
Forward primer (5'-3') AGCTAACCGCGGATGAGCTGAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGAGAACAGCCATTTCTTTCTCAG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 230	Approximate temperature 55°C

PCR data Exp. ID 10657
Forward primer (5'-3') AGCTAACCGCGGATGAGCTGAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGAGAACAGCCATTTCTTTCTCAG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 230	Approximate temperature 55°C

PCR data Exp. ID 10658
Forward primer (5'-3') AGCTAACCGCGGATGAGCTGAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGAGAACAGCCATTTCTTTCTCAG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 230	Approximate temperature 55°C

PCR data Exp. ID 32242
Forward primer (5'-3') AGCTAACCGCGGATGAGCTGAAG	Digested band sizes (using XmnI) Solanum pennellii LA716: 90+140 Solanum lycopersicum LA925: 230	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTGAGAACAGCCATTTCTTTCTCAG	Enzymes All possible enzymes for pennellii and and lycopersicum: XmnI
Accessions and product sizes Solanum pennellii LA716: 230 Solanum lycopersicum LA925: 230	Approximate temperature 55°C