Sol Genomics Network

SGN Marker C2_At2g42750

SGN-M7500
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	308948 (SGN) 131584 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U197776	-	-	-
Lycopersicon Combined	Single	SGN-U222096	-	-	-
Arabidopsis	Single	At2g42750 on TAIR	-	-	-

Mapped locations

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46644)
Chromosome 7
Position 9.40 cM
Confidence F(LOD3)
Protocol dCAPS

Tobacco N. tomentosiformis

Map: Eggplant-COSII
Map version 63 (location: 42204)
Chromosome 7
Position 81.00 cM
Confidence F(LOD3)
Protocol CAPS

Eggplant-COSII

PCR data Exp. ID 43770

Forward primer (5'-3')

AAGAAGGCATACTATAGTTGCATGAA

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

ACTCTAGCTCTTCCAAAGTCTTCCTC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Pepper-COSII
Map version 58 (location: 41392)
Chromosome 7
Position 86.50 cM
Confidence I(LOD2)
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43368

Forward primer (5'-3')

TCCAGTGCAAAGGAGAGTTTATGATG

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

ACTCTAGCTCTTCCAAAGTCTTCCTC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38387)
Chromosome 7
Position 44.30 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39636

Forward primer (5'-3')

TCCAGTGCAAAGGAGAGTTTATGATG

Accessions and product sizes

Solanum lycopersicum TA209

1650

Solanum pennellii TA56

1650

Approximate temperature

55°C

Reverse primer (5'-3')

ACTCTAGCTCTTCCAAAGTCTTCCTC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

950+700

Solanum pennellii TA56

1650

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 25923)
Chromosome 2
Position 17.80 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10848
Forward primer (5'-3') TCCAGTGCAAAGGAGAGTTTATGATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTCTAGCTCTTCCAAAGTCTTCCTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 650	Approximate temperature 55°C

PCR data Exp. ID 10849
Forward primer (5'-3') TCCAGTGCAAAGGAGAGTTTATGATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTCTAGCTCTTCCAAAGTCTTCCTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 650	Approximate temperature 55°C

PCR data Exp. ID 10850
Forward primer (5'-3') TCCAGTGCAAAGGAGAGTTTATGATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTCTAGCTCTTCCAAAGTCTTCCTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 650	Approximate temperature 55°C

PCR data Exp. ID 10851
Forward primer (5'-3') TCCAGTGCAAAGGAGAGTTTATGATG	Digested band sizes (using TaqI) Solanum pennellii LA716: 520 Solanum lycopersicum LA925: 500	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACTCTAGCTCTTCCAAAGTCTTCCTC	Enzymes All possible enzymes for pennellii and and lycopersicum: AdeI/DraIII,AluI,BclI,DpnII,HincII,Hpy188I,RsaI,TaqI All possible enzymes for lycopersicum and pimpinellifolium: AdeI/DraIII,BstUI,HincII,Hpy188I,RsaI
Accessions and product sizes Solanum pennellii LA716: 650 Solanum lycopersicum LA925: 650	Approximate temperature 55°C