Sol Genomics Network

SGN-M7535
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	302296 (SGN) 124295 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U223440	-	-	-
Solanum tuberosum	Single	SGN-U258539	-	-	-
Arabidopsis	Single	At2g45730 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39643

Forward primer (5'-3')

TCCATTCGGGTCTCTGTTTCAAG

Accessions and product sizes

Solanum lycopersicum TA209

600

Solanum pennellii TA56

600

Approximate temperature

55°C

Reverse primer (5'-3')

TTTCGCATCTCGTCAATATCTTC

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

150

Solanum lycopersicum TA209

290

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 10875
Forward primer (5'-3') TCCATTCGGGTCTCTGTTTCAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTCGCATCTCGTCAATATCTTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1300	Approximate temperature 55°C

PCR data Exp. ID 10876
Forward primer (5'-3') TCCATTCGGGTCTCTGTTTCAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTCGCATCTCGTCAATATCTTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1250	Approximate temperature 55°C

PCR data Exp. ID 10877
Forward primer (5'-3') TCCATTCGGGTCTCTGTTTCAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTCGCATCTCGTCAATATCTTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1300	Approximate temperature 55°C

PCR data Exp. ID 13101
Forward primer (5'-3') TCCATTCGGGTCTCTGTTTCAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTCGCATCTCGTCAATATCTTC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 1000	Approximate temperature 55°C

PCR data Exp. ID 13102
Forward primer (5'-3') TCCATTCGGGTCTCTGTTTCAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTCGCATCTCGTCAATATCTTC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1000	Approximate temperature 55°C

PCR data Exp. ID 13103
Forward primer (5'-3') TCCATTCGGGTCTCTGTTTCAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTCGCATCTCGTCAATATCTTC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1000	Approximate temperature 55°C

PCR data Exp. ID 13104
Forward primer (5'-3') TCCATTCGGGTCTCTGTTTCAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTCGCATCTCGTCAATATCTTC	Enzymes
Accessions and product sizes Nicotiana plumbaginifolia TA3354: 1000	Approximate temperature 55°C

PCR data Exp. ID 32626
Forward primer (5'-3') TCCATTCGGGTCTCTGTTTCAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTCGCATCTCGTCAATATCTTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1500	Approximate temperature 55°C

PCR data Exp. ID 32975
Forward primer (5'-3') TCCATTCGGGTCTCTGTTTCAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTCGCATCTCGTCAATATCTTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1500	Approximate temperature 55°C

PCR data Exp. ID 32976
Forward primer (5'-3') TCCATTCGGGTCTCTGTTTCAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTCGCATCTCGTCAATATCTTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1500	Approximate temperature 55°C

PCR data Exp. ID 32977
Forward primer (5'-3') TCCATTCGGGTCTCTGTTTCAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTCGCATCTCGTCAATATCTTC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 1000	Approximate temperature 55°C

PCR data Exp. ID 32978
Forward primer (5'-3') TCCATTCGGGTCTCTGTTTCAAG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTCGCATCTCGTCAATATCTTC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 1000	Approximate temperature 55°C

PCR data Exp. ID 10878
Forward primer (5'-3') TCCATTCGGGTCTCTGTTTCAAG	Digested band sizes (using amplicon difference) Solanum pennellii LA716: 1300 Solanum lycopersicum LA925: 1250	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TTTCGCATCTCGTCAATATCTTC	Enzymes All possible enzymes for pennellii and and lycopersicum: amplicon difference All possible enzymes for lycopersicum and pimpinellifolium: amplicon difference
Accessions and product sizes Solanum pennellii LA716: 1300 Solanum lycopersicum LA925: 1250	Approximate temperature 55°C