Sol Genomics Network

SGN-M8328
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	303993 (SGN) 124633 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U197962	-	-	-
Lycopersicon Combined	Single	SGN-U217439	-	-	-
Solanum tuberosum	Single	SGN-U251403	-	-	-
Arabidopsis	Single	At4g22200 on TAIR	-	-	-

Tomato-EXPEN 2000

PCR data Exp. ID 39845

Forward primer (5'-3')

CACCAGACGAGTTGTACATCATAGTGCC

Accessions and product sizes

Approximate temperature

Unknown

Reverse primer (5'-3')

AGTTCTTCAAGGAAAGTAGCATTACC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11457
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 3mM
Reverse primer (5'-3') AGGTATGTACTCAGCCTTCATATCTGC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 150	Approximate temperature 55°C

PCR data Exp. ID 11458
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 3mM
Reverse primer (5'-3') AGGTATGTACTCAGCCTTCATATCTGC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 150	Approximate temperature 55°C

PCR data Exp. ID 11459
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 3mM
Reverse primer (5'-3') AGGTATGTACTCAGCCTTCATATCTGC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 150	Approximate temperature 55°C

PCR data Exp. ID 12319
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 700	Approximate temperature 55°C

PCR data Exp. ID 12320
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Solanum tuberosum TA3427: 700	Approximate temperature 55°C

PCR data Exp. ID 12321
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Solanum melongena TA2986: 700	Approximate temperature 55°C

PCR data Exp. ID 12322
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Capsicum annuum TA3428: 700	Approximate temperature 55°C

PCR data Exp. ID 12323
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 700	Approximate temperature 55°C

PCR data Exp. ID 12324
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 700	Approximate temperature 55°C

PCR data Exp. ID 12325
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 700	Approximate temperature 55°C

PCR data Exp. ID 12326
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 700	Approximate temperature 55°C

PCR data Exp. ID 12327
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Physalis TA1367: 700	Approximate temperature 55°C

PCR data Exp. ID 12328
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Petunia axillaris subsp. parodii TA3310: 700	Approximate temperature 55°C

PCR data Exp. ID 12329
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Petunia x hybrida TA3311: 700	Approximate temperature 55°C

PCR data Exp. ID 12330
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Coffea canephora BP409: 700	Approximate temperature 55°C

PCR data Exp. ID 33360
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3346: 700	Approximate temperature 55°C

PCR data Exp. ID 33361
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Nicotiana sylvestris TA3347: 700	Approximate temperature 55°C

PCR data Exp. ID 33362
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Nicotiana tomentosiformis TA3349: 700	Approximate temperature 55°C

PCR data Exp. ID 33363
Forward primer (5'-3') TTAAAGGTGTCACAAAGGAAATTCTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') AGTTCTTCAAGGAAAGTAGCATTACC	Enzymes
Accessions and product sizes Nicotiana otophora TA3350: 700	Approximate temperature 55°C