Sol Genomics Network

SGN-M8407
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	308360 (SGN) 129291 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U219086	-	-	-
Solanum tuberosum	Single	SGN-U247948	-	-	-
Arabidopsis	Single	At4g30220 on TAIR	-	-	-

Pepper-COSII

PCR data Exp. ID 43464

Forward primer (5'-3')

ACTGGAAAGCCTGTGATGGTAAAGC

Accessions and product sizes

Approximate temperature

58°C

Reverse primer (5'-3')

ATCAGTGAATTCTTCTGCATTTGC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Tomato-EXPEN 2000

PCR data Exp. ID 39863

Forward primer (5'-3')

ACTGGAAAGCCTGTGATGGTAAAGC

Accessions and product sizes

Solanum lycopersicum TA209

700

Solanum pennellii TA56

700

Approximate temperature

55°C

Reverse primer (5'-3')

TGCAAGTTCATATATGAATCCACAGAGAC

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11519
Forward primer (5'-3') ACTGGAAAGCCTGTGATGGTAAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCAAGTTCATATATGAATCCACAGAGAC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 200	Approximate temperature 55°C

PCR data Exp. ID 11520
Forward primer (5'-3') ACTGGAAAGCCTGTGATGGTAAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCAAGTTCATATATGAATCCACAGAGAC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 200	Approximate temperature 55°C

PCR data Exp. ID 11521
Forward primer (5'-3') ACTGGAAAGCCTGTGATGGTAAAGC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCAAGTTCATATATGAATCCACAGAGAC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 200	Approximate temperature 55°C

PCR data Exp. ID 32510
Forward primer (5'-3') ACTGGAAAGCCTGTGATGGTAAAGC	Digested band sizes (using HinfI) Solanum pennellii LA716: 120 Solanum lycopersicum LA925: 200	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') TGCAAGTTCATATATGAATCCACAGAGAC	Enzymes All possible enzymes for pennellii and and lycopersicum: HinfI All possible enzymes for lycopersicum and pimpinellifolium: HinfI
Accessions and product sizes Solanum pennellii LA716: 200 Solanum lycopersicum LA925: 200	Approximate temperature 55°C