Sol Genomics Network

SGN-M8449
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	310260 (SGN) 129012 (CGN)	-	-	-
Lycopersicon Combined	Single	SGN-U218955	-	-	-
Solanum tuberosum	Single	SGN-U252109	-	-	-
Arabidopsis	Single	At4g33210 on TAIR	-	-	-

Arabidopsis COSII

PCR data Exp. ID 41620

Forward primer (5'-3')

TTGCCGTGCTTTAATTTCTCTTG

Accessions and product sizes

Solanum lycopersicum TA209

1850

Solanum pennellii TA56

1850

Approximate temperature

55°C

Reverse primer (5'-3')

ACAGGGGGCAATTAATAGATGCTTC

Digested band sizes (using amplicon difference)

Solanum lycopersicum TA209

650+1000

Solanum pennellii TA56

840+1000

Mg⁺² concentration

1.5mM

Enzymes

Other PCR data

PCR data Exp. ID 11545
Forward primer (5'-3') TTGCCGTGCTTTAATTTCTCTTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACAGGGGGCAATTAATAGATGCTTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 400	Approximate temperature 55°C

PCR data Exp. ID 11546
Forward primer (5'-3') TTGCCGTGCTTTAATTTCTCTTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACAGGGGGCAATTAATAGATGCTTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 400	Approximate temperature 55°C

PCR data Exp. ID 11547
Forward primer (5'-3') TTGCCGTGCTTTAATTTCTCTTG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACAGGGGGCAATTAATAGATGCTTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 400	Approximate temperature 55°C