Sol Genomics Network

SGN-M8724
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	302890 (SGN) 122519 (CGN)	-	-	-
Capsicum annuum	Single	SGN-U204273	-	-	-
Lycopersicon Combined	Single	SGN-U229597	-	-	-
Solanum tuberosum	Single	SGN-U263972	-	-	-
Arabidopsis	Single	At5g16620 on TAIR	-	-	-

Arabidopsis COSII

PCR data Exp. ID 41719

Forward primer (5'-3')

TGGAAACCCAGAATGGGACAACC

Accessions and product sizes

Solanum pennellii TA56

1000

Solanum lycopersicum TA209

1000

Approximate temperature

55°C

Reverse primer (5'-3')

ACCTGACACCCCAGGGAATAGTTC

Digested band sizes (using amplicon difference)

Solanum pennellii TA56

1000

Solanum lycopersicum TA209

750

Mg⁺² concentration

1.5mM

Enzymes

Other PCR data

PCR data Exp. ID 11744
Forward primer (5'-3') TGGAAACCCAGAATGGGACAACC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTGACACCCCAGGGAATAGTTC	Enzymes
Accessions and product sizes Solanum pennellii LA716: 1350	Approximate temperature 55°C

PCR data Exp. ID 11745
Forward primer (5'-3') TGGAAACCCAGAATGGGACAACC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTGACACCCCAGGGAATAGTTC	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 1350	Approximate temperature 55°C

PCR data Exp. ID 11746
Forward primer (5'-3') TGGAAACCCAGAATGGGACAACC		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ACCTGACACCCCAGGGAATAGTTC	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 1350	Approximate temperature 55°C