Sol Genomics Network

SGN Marker C2_At5g41350

SGN-M8923
COSII markers are Conserved Ortholog Set II - orthologs between many asterid species

Synonyms

None

Locus associations

None

Orthologs in this COSII group

Species	Copies	Sequence ID	CDS/Edited sequence	Peptide sequence	Predicted introns
Coffee	Single	300464 (SGN) 123271 (CGN)	-	-	-
Lycopersicon Combined	Multiple	SGN-U222128	-	-	-
Solanum tuberosum	Single	SGN-U266452	-	-	-
Arabidopsis	Single	At5g41350 on TAIR	-	-	-

Mapped locations

Map: Tobacco N. tomentosiformis
Map version 91 (location: 46768)
Chromosome 8
Position 77.00 cM
Confidence F(LOD3)
Protocol CAPS

Tobacco N. tomentosiformis

Map: Eggplant-COSII
Map version 63 (location: 42255)
Chromosome 8
Position 110.40 cM
Confidence F(LOD3)
Protocol dCAPS

Eggplant-COSII

PCR data Exp. ID 43824

Forward primer (5'-3')

TGAACTTGAGAAGTCTGAGGAACTCG

Accessions and product sizes

Approximate temperature

55°C

Reverse primer (5'-3')

ATGAAACTGATGCTCGCATTTTG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Pepper-COSII
Map version 58 (location: 41533)
Chromosome 1
Position 8.60 cM
Confidence F(LOD3)
Protocol CAPS

Pepper-COSII

PCR data Exp. ID 43509

Forward primer (5'-3')

ACCTCCAATGCCAATGCCTTATG

Accessions and product sizes

Approximate temperature

53°C

Reverse primer (5'-3')

ATGAAACTGATGCTCGCATTTTG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

Unknown

Enzymes

Map: Tomato-EXPEN 2000
Map version 52 (location: 38858)
Chromosome 8
Position 75.50 cM
Confidence I
Protocol CAPS

Tomato-EXPEN 2000

PCR data Exp. ID 39965

Forward primer (5'-3')

ACCTCCAATGCCAATGCCTTATG

Accessions and product sizes

Solanum lycopersicum TA209

250

Solanum pennellii TA56

250

Approximate temperature

53°C

Reverse primer (5'-3')

ATGAAACTGATGCTCGCATTTTG

Digested band sizes (using unknown enzyme)

Mg⁺² concentration

1.5mM

Enzymes

Map: Arabidopsis COSII
Map version 44 (location: 27388)
Chromosome 5
Position 16.56 MB
Confidence uncalculated
Protocol CAPS

Arabidopsis COSII

Other PCR data

PCR data Exp. ID 11891
Forward primer (5'-3') ACCTCCAATGCCAATGCCTTATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGAAACTGATGCTCGCATTTTG	Enzymes
Accessions and product sizes Solanum pennellii LA716: 430	Approximate temperature 55°C

PCR data Exp. ID 11892
Forward primer (5'-3') ACCTCCAATGCCAATGCCTTATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGAAACTGATGCTCGCATTTTG	Enzymes
Accessions and product sizes Solanum lycopersicum LA925: 430	Approximate temperature 55°C

PCR data Exp. ID 11893
Forward primer (5'-3') ACCTCCAATGCCAATGCCTTATG		Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGAAACTGATGCTCGCATTTTG	Enzymes
Accessions and product sizes Solanum pimpinellifolium LA1589: 430	Approximate temperature 55°C

PCR data Exp. ID 11894
Forward primer (5'-3') ACCTCCAATGCCAATGCCTTATG	Digested band sizes (using TaqI) Solanum pennellii LA716: 430 Solanum lycopersicum LA925: 230	Mg⁺² concentration 1.5mM
Reverse primer (5'-3') ATGAAACTGATGCTCGCATTTTG	Enzymes All possible enzymes for pennellii and and lycopersicum: BsoBI,BsrGI,TaqI,XhoI All possible enzymes for lycopersicum and pimpinellifolium: BsrGI
Accessions and product sizes Solanum pennellii LA716: 430 Solanum lycopersicum LA925: 430	Approximate temperature 55°C