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Tobacco locus pathogenesis-related protein 1

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Created on: 2008-09-02

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Literature annotations [11]

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Changes in chromatin structure due to hypomethylation induced with 5-azacytidine or DL-ethionine. FEBS letters (1992)
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Changes in chromatin structure of the HRS60 family of repetitive sequences in tobacco DNA were studied after hypomethylation induced with 5-azacytidine or DL-ethionine. The TaqI site in the HRS60 units lies in nucleosomal core regions and its cleavage is enhanced in the hypomethylated chromatin. In contrast, the cleavage of the Sau3AI site located in linker DNA does not depend on the level of methylation of DNA.

Fajkus, J. Vyskot, B. Bezdĕk, M. FEBS letters. 1992. 314(1). 13-6.

The nucleotide sequence of pathogenesis-related (PR) 1b protein gene of tobacco. Nucleic acids research (1990)
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Ohshima, M. Harada, N. Matsuoka, M. Ohashi, Y. Nucleic acids research. 1990. 18(1). 181.

The nucleotide sequence of pathogenesis-related (PR) 1c protein gene of tobacco. Nucleic acids research (1990)
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Ohshima, M. Harada, N. Matsuoka, M. Ohashi, Y. Nucleic acids research. 1990. 18(1). 182.

Nucleotide sequences of two PR-1 pseudogenes from Nicotiana tabacum cv. Wisconsin 38. Nucleic acids research (1990)
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Pfitzner, AJ. Pfitzner, UM. Goodman, HM. Nucleic acids research. 1990. 18(11). 3404.

Isolation and sequencing of HRS60dim1, a dimeric member of the HRS60-family of a tobacco DNA repeat. Nucleic acids research (1989)
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Matyásek, R. oukalová, B. Reich, J. Nucleic acids research. 1989. 17(11). 4377.

Isolation and nucleotide sequence of cDNA clones for the pathogenesis-related proteins PR1a, PR1b and PR1c of Nicotiana tabacum cv. Xanthi nc induced by TMV infection. Nucleic acids research (1988)
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Cutt, JR. Dixon, DC. Carr, JP. Klessig, DF. Nucleic acids research. 1988. 16(20). 9861.

Isolation and characterization of cDNA clones encoding pathogenesis-related proteins from tobacco mosaic virus infected tobacco plants. Nucleic acids research (1987)
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Infection of the tobacco cultivar Samsun NN by tobacco mosaic virus (TMV) results in a hypersensitive response. During this defense reaction several host encoded proteins, known as pathogenesis-related proteins (PR-proteins), are induced. Poly(A)+ RNA from TMV infected tobacco plants was used to construct a cDNA library. Thirty two cDNA clones were isolated and after digestion with different restriction endonucleases, twenty clones were found to code for PR-1a, six clones for PR-1b, and four clones for PR-1c. Two independent cDNA clones of each class were further characterized by DNA sequence analysis. All clones analyzed contained the 138 amino acid coding regions of their respective mature proteins, but only partial sequences of the signal peptides. Minor differences between the nucleotide sequences for clones belonging to the same class were detected. Comparison of the amino acid sequence for PR-1a deduced from its nucleotide sequence with published data obtained by Edman degradation of the protein showed four differences. Analysis of the 3' ends of the cDNA clones indicates that various alternate poly(dA) addition sites are used. Southern blot analysis using these cDNAs as probes suggests the presence of multiple PR-protein genes in the genomes of tobacco and tomato plants.

Pfitzner, UM. Goodman, HM. Nucleic acids research. 1987. 15(11). 4449-65.

Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group. Nucleic acids research (1987)
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Infection of Samsun NN tobacco with tobacco mosaic virus (TMV) was found to induce the synthesis of mRNA encoding a basic protein with a 67% amino acid sequence homology to the known acidic pathogenesis-related (PR) proteins 1a, 1b and 1c. By Southern blot hybridization it was shown that the tobacco genome contains at least eight genes for acidic PR-1 proteins and a similar number of genes encoding the basic homologues. Clones corresponding to three of the genes for acidic PR-1 proteins were isolated from a genomic library of Samsun NN tobacco. The nucleotide sequence of these genes and their flanking sequences were determined. One clone was found to correspond to the PR-1a gene; the two other clones do not correspond to known TMV-induced PR-1 mRNA's and may represent silent genes. Compared to the PR-1a gene, these genes contain an insertion or deletion in the putative promoter region and mutations affecting the PR-1 reading frame.

Cornelissen, BJ. Horowitz, J. van Kan, JA. Goldberg, RB. Bol, JF. Nucleic acids research. 1987. 15(17). 6799-811.

Nucleotide sequence of the PR-1 gene of Nicotiana tabacum. FEBS letters (1987)
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A gene encoding one of the pathogenesis-related proteins, PR1a, and two related pseudogenes were isolated from Nicotiana tabacum. The cloned PR1a gene (pPR-gamma) and one of the pseudogenes (pPR-alpha) were sequenced and found to have similar structures. The sequence of pPR-gamma was quite similar to that of the cDNA clone of PR1a. The plasmid pPR-gamma did not contain an intron and had a typical promoter sequence in the 5'-flanking region.

Ohshima, M. Matsuoka, M. Yamamoto, N. Tanaka, Y. Kano-Murakami, Y. Ozeki, Y. Kato, A. Harada, N. Ohashi, Y. FEBS letters. 1987. 225(1-2). 243-6.

Molecular characterization of messenger RNAs for ;pathogenesis-related' proteins 1a, 1b and 1c, induced by TMV infection of tobacco. The EMBO journal (1986)
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A cDNA library was made to poly(A)-containing RNA from tobacco mosaic virus (TMV)-infected Samsun NN tobacco plants and clones corresponding to mRNAs for the ;pathogenesis-related' (PR) proteins 1a, 1b and 1c were identified. One clone was found to contain a complete copy of PR-1b mRNA. The structural organization of this RNA is: a leader sequence of 29 nucleotides, an open reading frame of 504 nucleotides encoding a 30 amino acid long signal peptide and a 138 amino acid long mature protein, and a 3'-non-coding region of 235 nucleotides. Two other clones were found to contain partial copies of PR-1a and PR-1c mRNAs. The data indicate an approximately 90% homology between the amino acid sequences of PR-1a, -1b and -1c. Using one of the clones as probe it was shown that in the TMV-inoculated lower leaves and the non-inoculated upper leaves of a tobacco plant, the PR-1 mRNAs become detectable from 2 and 8 days after inoculation, respectively.

Cornelissen, BJ. van Huijsduijnen, RA. Van Loon, LC. Bol, JF. The EMBO journal. 1986. 5(1). 37-40.

Classification and Structural Comparison of Full-Length cDNAs for Pathogenesis-Related Proteins. Plant physiology (1987)
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Fourteen cDNA clones of pathogenesis-related (PR) proteins, PR1a and PR1b of tobacco were obtained and classified into six groups based on restriction enzyme maps. To assign the groups to different classes of PR1 proteins, all the clones were partially sequenced and compared with amino acid sequences of PR1a and PR1b. Two groups of these corresponded to PR1a and four to PR1b. The results indicate that there are at least two kinds of PR1a mRNAs and four kinds of PR1b mRNAs. In fact, one cDNA insert hybridized to at least six to seven DNA fragments in restriction enzyme fragments of Samsun NN genomic DNA, indicating that the PR1 protein genes exist as a multigene family in the tobacco genome. Two sequences of essentially full-length cDNAs for PR1a and PR1b were determined and compared. The coding sequences of two cDNAs share 93% homology and the deduced amino acid sequences of PR1a and PR1b precursors, which are synthesized as larger precursors containing signal peptides, are 91% homologous. The homology of mature PR1a and PR1b regions is higher than that of larger precursors, 94% in the nucleotide sequence and 93% in the amino acid sequence, whereas that of the signal peptide regions is 80 and 90%, respectively. The hydropathy patterns and the secondary structures predicted by Chou-Fasman rules are similar to tomato PR protein in the half-side of the C terminus, which suggests that the half-C terminus side is important for the function of PR1 proteins.

Matsuoka, M. Yamamoto, N. Kano-Murakami, Y. Tanaka, Y. Ozeki, Y. Hirano, H. Kagawa, H. Oshima, M. Ohashi, Y. Plant physiology. 1987. 85(4). 942-946.

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