Clone Read LE_HBa0144B12_T7_100295

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0144B12_T7_100295
Primer
T7
ID from Seq. Facility
LE_HBa_144_B12-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
780 trimmed for vector and quality, 834 raw
Genbank Accession
DU091247
Sequence
>LE_HBa0144B12_T7_100295 (raw untrimmed)
GGCAGGCTGGGTCCCGGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTATTAACTCATTAACAAGCCTTAATGTTGTATCCTTGTCCCTAAGCA
TTGTCTTCATCATGAGAATGGATTGAGTTTATTGACAATGCCGAACCGTCATTCTCAACTTTGTTTTTCTCCTTGAATCTAGCTCTTGGGATCTCCAGTC
TGCTAGATAAAGTTACCTCCATGATGACTTGTCCTAAGCTATAAATTCATTCCCTTAGATGATCTTTTAACTTTCTCTCTAGTTAGGCCTTTTTGTAAGT
GGATCCGACACATTATCCTTTGATTTTACATAGTCAATTCTGATAATTCCACTAGAGAGTAGTTTTCTAACAGTATCATGTCTATGTCGTATATGATGAG
ACTTTCCGTTATACGTCATGCTCTATGCCCTTCCTATTGCATCTTGACTGTGAAAGTGTATGCATACTAATGCCAATGGTTTGGGCCAAAATAGAATATC
TATCAAGAAATTTTGGAGTCTTTTAACTTATTCACTGACCTTATCTAGAGCACCTAGCTCAAAGATCATTGTAGAGCTAGCGATACATGTCTCTTTTGAA
ATATTTCTAAGAGACTGCTCCTCTACCAATAGTAAATACATATCCAATCGTGAATTTTACTTCATTCTTCCAATGATTCAATTTGTATCACTATGTTTTT
CAATATTGTTGGATATTGTTATAATGCAAGATATAGTTTTATGTATTATTTTAAATACTCCAAAACTCTATTTATTTTTATCTAATGAATTTATTTGGGA
TCACTTGTAAACGGACTCAGTTTAAAATAGCAAA
>LE_HBa0144B12_T7_100295 (trimmed for quality and cloning vector)
AGCTTTATTAACTCATTAACAAGCCTTAATGTTGTATCCTTGTCCCTAAGCATTGTCTTCATCATGAGAATGGATTGAGTTTATTGACAATGCCGAACCG
TCATTCTCAACTTTGTTTTTCTCCTTGAATCTAGCTCTTGGGATCTCCAGTCTGCTAGATAAAGTTACCTCCATGATGACTTGTCCTAAGCTATAAATTC
ATTCCCTTAGATGATCTTTTAACTTTCTCTCTAGTTAGGCCTTTTTGTAAGTGGATCCGACACATTATCCTTTGATTTTACATAGTCAATTCTGATAATT
CCACTAGAGAGTAGTTTTCTAACAGTATCATGTCTATGTCGTATATGATGAGACTTTCCGTTATACGTCATGCTCTATGCCCTTCCTATTGCATCTTGAC
TGTGAAAGTGTATGCATACTAATGCCAATGGTTTGGGCCAAAATAGAATATCTATCAAGAAATTTTGGAGTCTTTTAACTTATTCACTGACCTTATCTAG
AGCACCTAGCTCAAAGATCATTGTAGAGCTAGCGATACATGTCTCTTTTGAAATATTTCTAAGAGACTGCTCCTCTACCAATAGTAAATACATATCCAAT
CGTGAATTTTACTTCATTCTTCCAATGATTCAATTTGTATCACTATGTTTTTCAATATTGTTGGATATTGTTATAATGCAAGATATAGTTTTATGTATTA
TTTTAAATACTCCAAAACTCTATTTATTTTTATCTAATGAATTTATTTGGGATCACTTGTAAACGGACTCAGTTTAAAAT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.93856
Expected error %
0.059074
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAU89783.1| Score 142 E Value 6e-39 Identity 58.40% Align. Length 489bp (62.69%) Frame 3
putative retrovirus-related pol polyprotein-like [Solanum tuberosum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT38766.1| Score 127 E Value 4e-44 Identity 53.60% Align. Length 619bp (79.36%) Frame 2
putative polyprotein [Solanum demissum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0144B12
Clone
Clone name
hba-144b12
LE_HBa0144B12
C03HBa0144B12
P144B12
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found