Clone Read LE_HBa0179I20_SP6_125386

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0179I20_SP6_125386
Primer
SP6
ID from Seq. Facility
LE_HBa_179_I20-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
596 trimmed for vector and quality, 1040 raw
Genbank Accession
DU053105
Sequence
>LE_HBa0179I20_SP6_125386 (raw untrimmed)
CCCCGGTTTTACGCGAAGGGCTAAGACTCTGATCAATGGTTCCACAAACATGCAAAACATACTCACATATGGGACATCCTTTACATTATCCATCTCCTAG
GCTTCAACATCTCCCTCACCGTTTTGCCCAATAAAATTTTTCTCTAGAGTGTCCTTGGCTTCAACATATTTGGTTGTCATTCTCATCAATGACTGTAATT
GCAGACAACTTTCCATATATAGCAGGCAACTTCATTTCTTTGTAAACATCAAACACCTCGACCTTGTCATGAGCTCTCATTTTTAGCCTATTATCTGTTA
CGTCAATTAATGCACCTCTCATTGCTAGTAATGGTTGTTGTAAAATAAATGGGACCTGGGGATCAAGCTCAAAATTCAAAATGACAAAGTCCACTGGGAA
AATTAGAGTTTCCACTTGAACCAAGACATCTTCAATAACACCATCTGGCCTTGACATCGAATGATGAGCTAATTGCAACATGGAAGTAGGCATCAAATTG
ATACTTGCGCCCAAATCACACAAATCTCTTGCCTCCACACATTTGCCAATTTTTATTTGAAGAGTGAAACTCCTGGGATCTTTCAACTTCATTGGGAGTC
GATTCTAGATTCTAGAATTGCACTCTTCAGTGAGTGTCACTGTCGCATACTCAGCCAATCGACTTTTTGTTTGTCCCCACATATTTTCACATATTTTGGA
ATACTTTAGGAAAGCCCCTGCACATTTCAATTCACAGGCAATTACCTTTCAAACACTCCCTCCTCCTTTTGCTTTTTGAATTTTTGGGGGGAATGAAGGT
AAGAAATTTCACTTTGGGATTCCTCAATTACCCTTTTCCCCTTGATTCTACTTCTTTCCCTCATTTTCCTATTTCCCTCTACACTTTTTTCTGTGGTTGT
AATTTCTTCTATAGATTAATTTCCACCGATTCCTCCCCCTTACCTTACTTCTGGGTTGGGTTGTGTTTTGTCACTTACCCCCTTTGGGTGTTTGATCTAT
GCTATTTTATGATATCTAAGCTTTGTTTAATAATGTTAGG
>LE_HBa0179I20_SP6_125386 (trimmed for quality and cloning vector)
GACTCTGATCAATGGTTCCACAAACATGCAAAACATACTCACATATGGGACATCCTTTACATTATCCATCTCCTAGGCTTCAACATCTCCCTCACCGTTT
TGCCCAATAAAATTTTTCTCTAGAGTGTCCTTGGCTTCAACATATTTGGTTGTCATTCTCATCAATGACTGTAATTGCAGACAACTTTCCATATATAGCA
GGCAACTTCATTTCTTTGTAAACATCAAACACCTCGACCTTGTCATGAGCTCTCATTTTTAGCCTATTATCTGTTACGTCAATTAATGCACCTCTCATTG
CTAGTAATGGTTGTTGTAAAATAAATGGGACCTGGGGATCAAGCTCAAAATTCAAAATGACAAAGTCCACTGGGAAAATTAGAGTTTCCACTTGAACCAA
GACATCTTCAATAACACCATCTGGCCTTGACATCGAATGATGAGCTAATTGCAACATGGAAGTAGGCATCAAATTGATACTTGCGCCCAAATCACACAAA
TCTCTTGCCTCCACACATTTGCCAATTTTTATTTGAAGAGTGAAACTCCTGGGATCTTTCAACTTCATTGGGAGTCGATTCTAGATTCTAGAATTG

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.947311
Expected error %
0.3418
Sequence Annotations 
Automatic Annotations
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF24531.1| Score 96 E Value 8e-19 Identity 13.90% Align. Length 416bp (69.80%) Frame 3
F7F22.17 [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF24529.1| Score 96 E Value 8e-19 Identity 13.90% Align. Length 416bp (69.80%) Frame 3
F7F22.15 [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0179I20
Clone
Clone name
hba-179i20
LE_HBa0179I20
P179I20
Clone type
BAC
Contamination
none
Estimated length
92,001
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found