Clone Read LE_HBa0054N10_T7_12547

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0054N10_T7_12547
Primer
T7
ID from Seq. Facility
LE_HBa_054_N10-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
577 trimmed for vector and quality, 806 raw
Genbank Accession
DU031276
Sequence
>LE_HBa0054N10_T7_12547 (raw untrimmed)
CTCGTTCTCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCTTCTTCTTCCAAGGCAGTCCATTTTTCCAAAATATTAGACCTTTTGGCTTTA
CTTCTGTTACTCAAAATTTGAGTTTTTTTACTGAGAATGATGCCACAACTAGGAATGCCAAGGGAAAGGAAGTGTCAATTGTTGAAAATGGTGTAGCAAG
TTATAATGAGGAGCTTAGTAGTGTTGATCATAGGAAAGAGTTGGATTTGGGTGGTGGAGATGATAAAGCAAGAAATATCAAACAATGGGGTAAAGATTGG
TGGTGGAAACAGGATGGTAGTGGTGAACTATGTAGCAAAGATTATGTAAAGGAGTGGATTGGTAGCCAGATATGTCCATCAACTAATCCTGAGTGGGACT
TAGAAAAGAAATGTTCACCCCAGAAAACCAGTTTAGATATTTCAGCACCACTGAGTAAACCTGAGGAAGTGCAAGAAACCACATTACAAGGACAAGAGCC
AGAAACTCTCAAGAAAGAGTCCGATAAGGAGATAACGGAGAAAACGTGTAATAAGAAGCCTAGAAAGATGAAAGAGTGGTGGAAAGAGGAGCATCTTGAT
GAACTAAGCAAGAAGAGTAAGAAGGCTAATAAACTAGAAAGTTTCGTAAGAAGCGGTTCAAGATTCCACCTTTTGATATTGGCAAACGCCTTCAATTTCC
AAAGAAAAAATGAAATTCTGAATGCCGTCTGATAATGGACATTTATCTTGATTCTTAAATTCACTTTTTCGGCAAACGGTTCGAAACATAATCCTTCTCC
CCTTTC
>LE_HBa0054N10_T7_12547 (trimmed for quality and cloning vector)
AGCTTCTTCTTCTTCCAAGGCAGTCCATTTTTCCAAAATATTAGACCTTTTGGCTTTACTTCTGTTACTCAAAATTTGAGTTTTTTTACTGAGAATGATG
CCACAACTAGGAATGCCAAGGGAAAGGAAGTGTCAATTGTTGAAAATGGTGTAGCAAGTTATAATGAGGAGCTTAGTAGTGTTGATCATAGGAAAGAGTT
GGATTTGGGTGGTGGAGATGATAAAGCAAGAAATATCAAACAATGGGGTAAAGATTGGTGGTGGAAACAGGATGGTAGTGGTGAACTATGTAGCAAAGAT
TATGTAAAGGAGTGGATTGGTAGCCAGATATGTCCATCAACTAATCCTGAGTGGGACTTAGAAAAGAAATGTTCACCCCAGAAAACCAGTTTAGATATTT
CAGCACCACTGAGTAAACCTGAGGAAGTGCAAGAAACCACATTACAAGGACAAGAGCCAGAAACTCTCAAGAAAGAGTCCGATAAGGAGATAACGGAGAA
AACGTGTAATAAGAAGCCTAGAAAGATGAAAGAGTGGTGGAAAGAGGAGCATCTTGATGAACTAAGCAAGAAGAGTA

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.945886
Expected error %
0.540891
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g80870.1 Score 79 E Value 2e-15 Identity 13.20% Align. Length 455bp (78.86%) Frame 2
68414.m09489 protein kinase family protein contains eukaryotic protein kinase domain, INTERPRO:IPR000719
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_178202.1| Score 79 E Value 9e-14 Identity 13.20% Align. Length 455bp (78.86%) Frame 2
protein kinase family protein [Arabidopsis thaliana] gb|AAF14675.1| Contains similarity to gb|U82481 KI domain interacting kinase 1 from Zea mays and contains PF|00069 Eukaryotic protein kinase domain. ESTs gb|H77140, gb|H76842 and gb|AI994303 come from this gene. [Arabidopsis thaliana] pir||E96841 hypothetical protein F23A5.23 [imported] - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_481204.1| Score 70 E Value 3e-11 Identity 13.80% Align. Length 296bp (51.30%) Frame 2
putative SERK1 protein [Oryza sativa (japonica cultivar-group)] dbj|BAC99478.1| putative SERK1 protein [Oryza sativa (japonica cultivar-group)] dbj|BAC99473.1| putative SERK1 protein [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0054N10
Clone
Clone name
hba-54n10
LE_HBa0054N10
C03HBa0054N10
P054N10
Clone type
BAC
Contamination
none
Estimated length
100,608
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found