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Clone Read LE_HBa0187O23_T7_126684

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0187O23_T7_126684
Primer
T7
ID from Seq. Facility
LE_HBa_187_O23-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
574 trimmed for vector and quality, 936 raw
Sequence
>LE_HBa0187O23_T7_126684 (raw untrimmed)
CTAAATTGGGTCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTCTTCAGACAATGGAGATTTCAGTGGTGAAGGACCTCCGAAGAAAGGG
GATGAAAAGAAAACATATACACAGCAGAAAACAAAGATGCAAAGAAGAGACACTTCACAGAAAAAAGAATTTGTTGGATGGGGATCAAAATCTCTAATTG
ACTTTCTTCAGTTTATAGGTCACGATACCAGAGAAAAATTATCCCCATATGATGTGACATCGATGGTAATTAAATATGCAAATGAAAATGACCTTATCCA
TCCTATTGAAAAGAGAAAAATCCTTTGTGATATTCAGTTAGAAGCTCTTTTGGGGGGGAAAGTGGTAAACAGAGACATAATACTTAGCCTCCTTAAAAGC
CATTTTGTTGAAAATGAGGAGCGATTACAAAAGAATGAGCTTGCTCATGATCTTGAAGACAATGACACAGAAATGTTTGTGGCTTCTAAAACTGAGAAAA
AGGTTGAACAAAACAAAAGATCCTCAATTTGGTATTCAACTGCAGCACAAAGTCAATTTGCAGCCTTGATTCCTGAAAATATCAAACTTGTTTACCTGAA
GAGGAGTTTGGTTCTGGAAATGATCAAGAAGCCTGAGTCGTTTGAAACCAAATTATAAGAAATTTTTGTTCGGGTAAGTTAAATTTCCGTGAATTTGAAG
CTCGCTAACTCCTCACAGCTTGGGGAAATTAAAGGGTGTTTAAACCAAAGAATTTTTTCTTTTATTTTTTTTTTTAAAAAGGTTAAATAACCCTCAATGG
ACGGTGGGACCAGGTGGCCAAGGGGAATTAGGATCTTGTGTTCGCCCCATAAATGATTGAAGTGCGCAATAATCTCCAAGACGATAGCGGTGATAGATGT
CAATGGGTGAGCTATGCGCGATGCTCAGACCAAGCA
>LE_HBa0187O23_T7_126684 (trimmed for quality and cloning vector)
AGCTTTCTTCAGACAATGGAGATTTCAGTGGTGAAGGACCTCCGAAGAAAGGGGATGAAAAGAAAACATATACACAGCAGAAAACAAAGATGCAAAGAAG
AGACACTTCACAGAAAAAAGAATTTGTTGGATGGGGATCAAAATCTCTAATTGACTTTCTTCAGTTTATAGGTCACGATACCAGAGAAAAATTATCCCCA
TATGATGTGACATCGATGGTAATTAAATATGCAAATGAAAATGACCTTATCCATCCTATTGAAAAGAGAAAAATCCTTTGTGATATTCAGTTAGAAGCTC
TTTTGGGGGGGAAAGTGGTAAACAGAGACATAATACTTAGCCTCCTTAAAAGCCATTTTGTTGAAAATGAGGAGCGATTACAAAAGAATGAGCTTGCTCA
TGATCTTGAAGACAATGACACAGAAATGTTTGTGGCTTCTAAAACTGAGAAAAAGGTTGAACAAAACAAAAGATCCTCAATTTGGTATTCAACTGCAGCA
CAAAGTCAATTTGCAGCCTTGATTCCTGAAAATATCAAACTTGTTTACCTGAAGAGGAGTTTGGTTCTGGAAAT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.93373
Expected error %
0.5605
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g08430.1 Score 101 E Value 2e-22 Identity 11.70% Align. Length 476bp (82.93%) Frame 1
68418.m00993 SWIB complex BAF60b domain-containing protein / plus-3 domain-containing protein / GYF domain-containing protein similar to CPRF interacting protein [Petroselinum crispum] GI:9588690; contains Pfam profiles PF02201: BAF60b domain of the SWIB complex, PF03126: Plus-3 domain, PF02213: GYF domain; contains non-conensus AT-AC splice sites at intron 5
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g63700.1 Score 95 E Value 2e-20 Identity 12.90% Align. Length 464bp (80.84%) Frame 1
68418.m07996 zinc finger (C3HC4 type RING finger) family protein contains Pfam PF03126: Plus-3 domain; contains Pfam PF02201: BAF60b domain of the SWIB complex; contains Pfam PF00628: PHD-finger domain; contains Prosite Zinc finger, C3HC4 type (RING finger), signature; similar to CPRF interacting protein (GI:9588690) [Petroselinum crispum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_196460.2| Score 101 E Value 1e-20 Identity 11.70% Align. Length 476bp (82.93%) Frame 1
SWIB complex BAF60b domain-containing protein / plus-3 domain-containing protein / GYF domain-containing protein [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAC08343.1| Score 101 E Value 1e-20 Identity 11.70% Align. Length 476bp (82.93%) Frame 1
putative protein [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0187O23
Clone
Clone name
hba-187o23
LE_HBa0187O23
P187O23
Clone type
BAC
Contamination
none
Estimated length
93,302
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found