Clone Read LE_HBa0001M12_SP6_127654

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0001M12_SP6_127654
Primer
SP6
ID from Seq. Facility
Le-HBa001_M12-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
5 trimmed for vector and quality, 1838 raw
Sequence
>LE_HBa0001M12_SP6_127654 (raw untrimmed)
CCCCCAAATCGAATACATTCCCCTTTTTAAAATAGGTGTCTCCCACTCGCGTTGCGTATTCTTCGCGCACTATCGTCCCTCTCCCCTATAAAAGAGGGGG
CGAGTCGTCCTATTTATGTCAGTACACTACATGGACAGCTGTTAGAGATCGGCGGATTGAGGGTTTCGGCCAGTTCCTCTCTTTTAAGAGGAGGCGTAGN
ATGATCTATTAGTTAGCTGCTTCACGATTGTGTGCGAACATGAGTTATTAGTACACCATAAACCACTGGAGTCGGTGGATAATGTATCTCAGTATACGAA
GATCCGTTGCGGGAGGGTACATAATAGTATAAATGCTTATTCAAACGCCGGCGATACCTCGAACGATTAGACCCTGATGCGTGGAGACTTCGCGCGTACA
CAGAATACGCAACCAACGATCAACAAGCTTTGTCTGACGATGCGACGATAAGTAGATTTGCGTTTAACAGCTCTTTGATAAACAAAAGTTATAAATCTCT
CACCCTACCATGACAGATATTAAACATGAGTTCTGCATACCACATACCCAAAACAATCCTGAACAAATTGATCTATCTAATCCCTGTCTTACATATCGTC
GTTTGCCCAACCCTAATTGCGTCGTCTAGGGGGCAATTTGTCCTTGACTGATTTTCTTTAATACTTCACATAAAAAGGCACACACATCTTGACACAACAC
AGATTATCAGAGGAACACACGTAAGGAGACACCGGATACAAAATACCTGCCACAATTGGGGCTCACTGAGGACATTTCGTCGATAGATTGTTTGCCCCTC
CTCAAGCCGGACCACTACACAGAACCAGTCACCTCCCTGGCATTCAAACGCTATCCGAATCACCCCAAAACGCCCGCTGCTCCGTCTAACCTCCTGCTAC
TATATGCCCCCACCCACCTGCAACACTGGGAATCACCGCATCTGCCCGTCGCATTGACTACACCAGCCCCTCCCAACCTCCCACCCTTCATCATAACTCC
CACACAACCAACTATCCACCATTCATCCCTCCTCGGTCTCATCCTCCTCCTAATAAACGATCTACGATGCATCCTAAGCCCGGATCATCGATTCCCACTC
CAACGCTCACCCTCCCAACCCCCTGCGCGCCGACCCAACCGCGGCTGCGCTCACACCCACGCCCCACGGGATCTTGGCCTATCGCTAGGCATGACCACTC
GCGCCCCGTGTTCTGCCTCACGCTTGCGTCACCTCCTCATCCATCCGCAATCTCCTGCAGTGGCTTAAAACATCACATGCTACGACAGGGATCCCGACAC
TGCGCAGCCCTCTTTACGATCACTACCTCTCAATAGCACTCATTAGAACGTCTAAATTATCATCGCACTCACTCTAAAATAATTACTCTCGCGCGTCGTT
GTCACTCCACCTAAATCATCTCAACCTCTACACACTGCCATCTTCACTACTCAGCCCCCTCACTCCCCGCCGCGCCTAACCGCATTCAATATGTCCTCCT
CCTGCTTTCATGCACCTTGTCCTTTGAGCCCACCATTAGCTACTGCCATCGGCTCTTACACAATTCACACTTTTGCTCAGCACAATGCTATCACTGTCTC
TCTCTACACTACTCGCCATGTACGCCACGGCAAACACATGTACCATCAGCTTCCATATCACACGTGCTCATGCGCAGCTAACTGCGATGCGAACGAGAGA
TCTCTCACTCTCACCTGATGCCCTCNCCCTACGATTCTTCGGCTCATGTTCATCTTGTTGCTACATGTTTGGTGAATTGTCGNTATGCNGCACGGACGTC
ATACTGCGANGTGACGCTGCACGCTTGTATGTGACATT
>LE_HBa0001M12_SP6_127654 (trimmed for quality and cloning vector)
AGGGT

This is probably not a useful sequence.

Problems:
  • Low complexity
  • Short cloning insert
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
not recorded
Expected error %
0.5632
Sequence Annotations 
Automatic Annotations
No automatic annotations found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0001M12
Clone
Clone name
hba-1m12
LE_HBa0001M12
C01HBa0001M12
P001M12
Clone type
BAC
Contamination
none
Estimated length
140,000
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
3 sequencing reads found