Clone Read LE_HBa0017M12_SP6_130763

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0017M12_SP6_130763
Primer
SP6
ID from Seq. Facility
LE_HBa_017_M12-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
757 trimmed for vector and quality, 942 raw
Genbank Accession
DU049949
Sequence
>LE_HBa0017M12_SP6_130763 (raw untrimmed)
TACCTTGGAAAAAGAAATATCGTCGAATCACATCATACTGTGTCTTTCTACCATAAAGATTGCTGCACTTATAATTCCAGGAGCATTCAGTACATCTTCC
TAAATTATGACATATTGTACATAAGATATGTGCAGACTCTGCATTTTCTAGTATGAAATTAAACAAACAGTGCATAGCCGCTACAACTCTTTGAAGACTT
AGTTTTCAGCAGAAAAACAGATATGTTTCGCATTGCTATTCGACATATGCGCCCACACAGAGAAAGAGAGAGAGAACTTACCAAATCTTCTCATTGCGAT
TCGACCGGAAGACCTTTCTCCAGATATAGTTTCATTAATTGCTTGAACCATCTCATTCCTGCTTACATAGCCATCCTTGTTCTTGTCTAAAAACACAAAA
GCATCAACAAGCGTTTCAAATGTTACCTCCAGATTTGGTAGTCCCATCCGCGATTTCTGTATGTGGTCAAGGAATAAACAACTTTGCTCGTATGGGGCTG
GGGGTAGGGGATCGGAACAACATGTTAACGTAGTTCCTCATGAAGGGCTACAGAATGCTAAGAAAATGGAATGCATCTCCACGAGAACAAGTAAGCAGAT
AATCTGTTGTCCGTACTCTCCAAAAAAATCGTCGTATTGGGTCGGATCCTTCAAAATGCACTACTTTTGGAGGATCTGATACATACTCAACAATATTTTT
GGAGAGTCCGCGCAACATAAAAGAGAACAATCTTTGAAAAGCACCATCTCACTTGAAAGTAATCAACTAATGTCGTTTTCTGAAAAAAAATGTTCTTTTC
CTACATTTCTGAAAAACATGAGTTAAAAAGAAAACTGTGAGCTGGATACCGCCTGCAAAGCAGAAAGATCATCCTTCAGAAGATAGACAAGACCAAAAGA
ACGAGGAATTCGCTGAACTCTATTCCATAACCTCATTAATAT
>LE_HBa0017M12_SP6_130763 (trimmed for quality and cloning vector)
TCTTTCTACCATAAAGATTGCTGCACTTATAATTCCAGGAGCATTCAGTACATCTTCCTAAATTATGACATATTGTACATAAGATATGTGCAGACTCTGC
ATTTTCTAGTATGAAATTAAACAAACAGTGCATAGCCGCTACAACTCTTTGAAGACTTAGTTTTCAGCAGAAAAACAGATATGTTTCGCATTGCTATTCG
ACATATGCGCCCACACAGAGAAAGAGAGAGAGAACTTACCAAATCTTCTCATTGCGATTCGACCGGAAGACCTTTCTCCAGATATAGTTTCATTAATTGC
TTGAACCATCTCATTCCTGCTTACATAGCCATCCTTGTTCTTGTCTAAAAACACAAAAGCATCAACAAGCGTTTCAAATGTTACCTCCAGATTTGGTAGT
CCCATCCGCGATTTCTGTATGTGGTCAAGGAATAAACAACTTTGCTCGTATGGGGCTGGGGGTAGGGGATCGGAACAACATGTTAACGTAGTTCCTCATG
AAGGGCTACAGAATGCTAAGAAAATGGAATGCATCTCCACGAGAACAAGTAAGCAGATAATCTGTTGTCCGTACTCTCCAAAAAAATCGTCGTATTGGGT
CGGATCCTTCAAAATGCACTACTTTTGGAGGATCTGATACATACTCAACAATATTTTTGGAGAGTCCGCGCAACATAAAAGAGAACAATCTTTGAAAAGC
ACCATCTCACTTGAAAGTAATCAACTAATGTCGTTTTCTGAAAAAAAATGTTCTTTT

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.969841
Expected error %
0.2949
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g26470.1 Score 89 E Value 3e-18 Identity 24.70% Align. Length 185bp (24.44%) Frame 2
68417.m03808 calcium-binding EF hand family protein low similarity to SP|P06787 Calmodulin {Saccharomyces cerevisiae}; contains INTERPRO:IPR002048 calcium-binding EF-hand domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|XP_464487.1| Score 99 E Value 2e-19 Identity 27.00% Align. Length 173bp (22.85%) Frame 2
calcium-binding EF hand-like protein [Oryza sativa (japonica cultivar-group)] dbj|BAD25460.1| calcium-binding EF hand-like protein [Oryza sativa (japonica cultivar-group)]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAD35476.1| Score 97 E Value 3e-19 Identity 27.00% Align. Length 173bp (22.85%) Frame 2
calcium-binding EF hand-like protein [Oryza sativa (japonica cultivar-group)] dbj|BAD35633.1| calcium-binding EF hand-like protein [Oryza sativa (japonica cultivar-group)]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0017M12
Clone
Clone name
hba-17m12
LE_HBa0017M12
P017M12
Clone type
BAC
Contamination
none
Estimated length
79,699
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found