Clone Read LE_HBa0022H14_T7_133600

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0022H14_T7_133600
Primer
T7
ID from Seq. Facility
LE_HBa_022_H14-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
670 trimmed for vector and quality, 963 raw
Genbank Accession
DU041257
Sequence
>LE_HBa0022H14_T7_133600 (raw untrimmed)
GTCAGTGGGGTCCGGGGGGTCCTTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGGTCGAGCCAGCTTGATCACAATTGAAGTACAAGAAAGTGAAAACG
CATTTTTAAAAGATGTGATTAAAAAATATTTTAATATATTTCACATATTTTTAGTTTAGAATTGTTAAATTTAAAAAAATCTTTATTTTTTGAGAGAACT
ACTTAAAGCACCTTTGAAGTTGGTGTAAATTATAAGTTTTATTTCTGAATTATTGACAACTTTTAAAACAACCCTCTACTTGACTAGCTAAACTTAGATA
CGCCCTCAATCTAATACGATAACGTAGTGAGTAATTTCAAATTTTTGTAGGAGTATGTAACTCTTAATAAAAACCCGGAAAAACATATTGAATTTAAAAT
ATTCCTTGATATATTTAAGTTTAGTAAATCAATAAAATAATCATTTTTAAGCCTGACAATAATTTTGAAATCACACTAATAATCCGTGCTTAACTTAAGG
ATATTTCAATACTTCTCTTATTTTTTAGGAAAATAGTTTGAAATATATCTGAACTTCTCTCTTATTTTTAACCTCCCTAAACTATTTAACAGTGTATTTT
AAAGGTATATATGTGCTCACGTGGACAAAAGAAATATTGCATAGTTACAAATAATAATGTGTCCACGTGGACATATATATACCTTTAATATAAACTATTA
AATAGTTCAGATAGGTAAAAAGTCCTCCATGAAATTTGGTATCGTAACAATAATTTCAACCAAAATTAAATAATTTTTCCGACGTTTTCCTTATTTTTTA
TAAAAACAAATGGAGTACTTAGAGTAGTTAGGATATAAACATACTCCCTGGAAAATGTGGATAATATATAATGGATATGAGGTGATAGAACTCCGCAGTT
GGGGGGGGTTGGGTTGGGACTACTATTTTTGTGCCCCAGGCCCCGGCTCCCCACCCTGAAACC
>LE_HBa0022H14_T7_133600 (trimmed for quality and cloning vector)
CTAGAGTCGACCTGCAGGCATGCAAGCTTGGGTCGAGCCAGCTTGATCACAATTGAAGTACAAGAAAGTGAAAACGCATTTTTAAAAGATGTGATTAAAA
AATATTTTAATATATTTCACATATTTTTAGTTTAGAATTGTTAAATTTAAAAAAATCTTTATTTTTTGAGAGAACTACTTAAAGCACCTTTGAAGTTGGT
GTAAATTATAAGTTTTATTTCTGAATTATTGACAACTTTTAAAACAACCCTCTACTTGACTAGCTAAACTTAGATACGCCCTCAATCTAATACGATAACG
TAGTGAGTAATTTCAAATTTTTGTAGGAGTATGTAACTCTTAATAAAAACCCGGAAAAACATATTGAATTTAAAATATTCCTTGATATATTTAAGTTTAG
TAAATCAATAAAATAATCATTTTTAAGCCTGACAATAATTTTGAAATCACACTAATAATCCGTGCTTAACTTAAGGATATTTCAATACTTCTCTTATTTT
TTAGGAAAATAGTTTGAAATATATCTGAACTTCTCTCTTATTTTTAACCTCCCTAAACTATTTAACAGTGTATTTTAAAGGTATATATGTGCTCACGTGG
ACAAAAGAAATATTGCATAGTTACAAATAATAATGTGTCCACGTGGACATATATATACCTTTAATATAAA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.914595
Expected error %
0.1798
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|L08952.1:1-181 Score 68 E Value 1e-12 Identity 100.00% Align. Length 33bp (4.93%) Frame 2
pT7T319 cloning vector
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|S52393.1:14-153 Score 62 E Value 8e-11 Identity 100.00% Align. Length 30bp (4.48%) Frame 2
{polylinker} [pIC20HE, Plasmid Recombinant, 153 nt]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0022H14
Clone
Clone name
hba-22h14
LE_HBa0022H14
C08HBa0022H14
P022H14
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found