Clone Read LE_HBa0024J22_T7_135248

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0024J22_T7_135248
Primer
T7
ID from Seq. Facility
LE_HBa_024_J22-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
815 trimmed for vector and quality, 964 raw
Genbank Accession
DU047175
Sequence
>LE_HBa0024J22_T7_135248 (raw untrimmed)
CTAACCCTGGTCCTGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCCCAACATCCCTTCCACAGTCTCAGGATTTGCACTTGCTCCAGAGCAC
TGCATTGACTGGCGACCGTACATGTAATATGTTCCTGAAGCTAGCCTCTTAGGGTCTAATAACGATATGCACACAGATAACGAGTTAACCAACACAGATT
TAGGTCGTGACTCCTCCAGAGCATGGCGAAATAACCTCTCAATGAAACTGTGGAACATCATATAACCAAATATGTTAGTGAGACCCGAGAAAATACAATA
GTATCACAGGACTAAGGCTTCAATCAAAATCCAAAATTAATGAAGATTCAGAATATTAGAACACCTAGGGCTAGAGATTTTGGCAGCAATCCCAGGAGGA
GCATATCGAGTAATGGCACACAAAGTTTCTGCTGCATTAGCATGCACTTCAGGGCAATCCTGCAATTACAGTGAATTCCATTACCATAGTAACCTACACC
AGCAATTACAAGATTTGTAGGGTACTCAGTCACTAACTAAATTAGATGAACACCAGACCATCAATATTTGATCCATCCCTACTGAGCACATTATGTTTAA
CATTTAAATCGATAAAATATAACCTTCTCATCAGCCCCAGCACCTTGGTCGCCTAGAAAGCTATCATCAGGCATGCAGTAATTCCTTTTTTCAGAATGGC
AGCATAAAGCAGGAATGCAGTACTATCTTAGTTCCCCAAGCCAGGTTAGATATCGAAACACACAAATCATGTAATGTCTAATGACTGGGAAAATTAGAAT
GTTAGTAAATAAATTCAGGAAAATGACATACTGATGAGCTAAACTTATCAACGATCATCTCCAGCACATTTGTATCTTCCAACCCTGCATGTTCTCCGAG
AGTTGCTATACAAATGTTCATCGTTGCCATTAAGCGGATTAAACCTTAAATCAAAACATTGGAC
>LE_HBa0024J22_T7_135248 (trimmed for quality and cloning vector)
AGCTTCCCAACATCCCTTCCACAGTCTCAGGATTTGCACTTGCTCCAGAGCACTGCATTGACTGGCGACCGTACATGTAATATGTTCCTGAAGCTAGCCT
CTTAGGGTCTAATAACGATATGCACACAGATAACGAGTTAACCAACACAGATTTAGGTCGTGACTCCTCCAGAGCATGGCGAAATAACCTCTCAATGAAA
CTGTGGAACATCATATAACCAAATATGTTAGTGAGACCCGAGAAAATACAATAGTATCACAGGACTAAGGCTTCAATCAAAATCCAAAATTAATGAAGAT
TCAGAATATTAGAACACCTAGGGCTAGAGATTTTGGCAGCAATCCCAGGAGGAGCATATCGAGTAATGGCACACAAAGTTTCTGCTGCATTAGCATGCAC
TTCAGGGCAATCCTGCAATTACAGTGAATTCCATTACCATAGTAACCTACACCAGCAATTACAAGATTTGTAGGGTACTCAGTCACTAACTAAATTAGAT
GAACACCAGACCATCAATATTTGATCCATCCCTACTGAGCACATTATGTTTAACATTTAAATCGATAAAATATAACCTTCTCATCAGCCCCAGCACCTTG
GTCGCCTAGAAAGCTATCATCAGGCATGCAGTAATTCCTTTTTTCAGAATGGCAGCATAAAGCAGGAATGCAGTACTATCTTAGTTCCCCAAGCCAGGTT
AGATATCGAAACACACAAATCATGTAATGTCTAATGACTGGGAAAATTAGAATGTTAGTAAATAAATTCAGGAAAATGACATACTGATGAGCTAAACTTA
TCAACGATCATCTCC

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.971769
Expected error %
0.3677
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g30470.1 Score 122 E Value 2e-28 Identity 16.80% Align. Length 410bp (50.31%) Frame 3
68414.m03724 SIT4 phosphatase-associated family protein contains similarity to copper chaperone homolog CCH GB:AAF15286 GI:6525011 from [Glycine max]; contains Pfam profile PF04499: SIT4 phosphatase-associated protein
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g28360.1 Score 79 E Value 4e-15 Identity 13.30% Align. Length 398bp (48.83%) Frame 3
68415.m03447 SIT4 phosphatase-associated family protein contains Pfam profile: PF04499 SIT4 phosphatase-associated protein
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_174335.4| Score 122 E Value 1e-26 Identity 16.80% Align. Length 410bp (50.31%) Frame 3
SIT4 phosphatase-associated family protein [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAF19748.1| Score 122 E Value 1e-26 Identity 16.80% Align. Length 410bp (50.31%) Frame 3
Contains similarity to gb|D21822 ORF from Lilium longiflorum. ESTs gb|T03978, gb|R89954, gb|AI995107, gb|T20998, gb|R65384 come from this gene. [Arabidopsis thaliana] pir||D86429 F26G16.7 protein - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0024J22
Clone
Clone name
hba-24j22
LE_HBa0024J22
C02HBa0024J22
P024J22
Clone type
BAC
Contamination
none
Estimated length
68,066
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found