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Clone Read LE_HBa0031J18_SP6_139847

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0031J18_SP6_139847
Primer
SP6
ID from Seq. Facility
LE_HBa_031_J18-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
676 trimmed for vector and quality, 951 raw
Genbank Accession
DU048530
Sequence
>LE_HBa0031J18_SP6_139847 (raw untrimmed)
GGACGGTATAAGACGTTGCGCTATGGGATTCGAATTTTCTCAGATCAGAGAAGGGCCTTCTAATGCATCAAAGAAAGTATGCACTAAAATTAAGTTCCCA
AACTAGTCTAAGTGTAGCTAATCCAGCTGGAACGTTACTCCAATTGACACTGACTCAAGAACTAACTACTAAGAAGTATGATGAGGTGACACAGGTGCCA
TAAGATGATCCACTAACTGATCAAAGATCTTATCAAAGGATAGTTGACAAACTACTCTATTTAACTATGACAAGGCCTGATGTTTCTTATAGAGTCCAAA
TATTGAGTCAATTTCTACGACAACAACCTAAGAAATCTCATATGACTACAACATTAAGAATTGTGAGATATGTGAAAAATCAACCAGGGCAAGGTCTATT
ACTTTCTAGTTATTCAGAAAATACTATCACTACATATTGTGATGTAGATTGGGCTTCCTGTCCTCTAACAAGAAATTCAATAACTAGTTACTTTGTCCAA
TATGGGGGTTCATTTGTTTCTTGGAAAATAAAGTAACAAACAATTGTTTCTCGACGTTTAATTGAAGTTGAATACACGAGCATCGCAACCACTGTAAAAA
AATCATATGGTTGAAAGGTCTAAATAGAGTCCAACATTGAAGTCTGACTACCTATCTACATGCATATTAATAGCAAAGATGCTATACAACTTGCAGTCAA
TCCAGTCTATCATGAGATAACTAAACACATTGAGATTGACTGTCACTTTATTAAAGATAAAATTACATAAGGTTTAGTGTCTACAAAGTACATTCCTACA
AAAGATCAACCGGGTGATTTGTTCACTAAGGACTCCCAAATTGCAACTTCAACATATCATCTCCCAGCTAGGAGTTCTTAAAAAATTCTCACTGCCCATC
TTGGAAGGGAGGGTTGAGAGTGAATAGGAAAAGGCGAATCAATGGATGAGT
>LE_HBa0031J18_SP6_139847 (trimmed for quality and cloning vector)
AGCTGGAACGTTACTCCAATTGACACTGACTCAAGAACTAACTACTAAGAAGTATGATGAGGTGACACAGGTGCCATAAGATGATCCACTAACTGATCAA
AGATCTTATCAAAGGATAGTTGACAAACTACTCTATTTAACTATGACAAGGCCTGATGTTTCTTATAGAGTCCAAATATTGAGTCAATTTCTACGACAAC
AACCTAAGAAATCTCATATGACTACAACATTAAGAATTGTGAGATATGTGAAAAATCAACCAGGGCAAGGTCTATTACTTTCTAGTTATTCAGAAAATAC
TATCACTACATATTGTGATGTAGATTGGGCTTCCTGTCCTCTAACAAGAAATTCAATAACTAGTTACTTTGTCCAATATGGGGGTTCATTTGTTTCTTGG
AAAATAAAGTAACAAACAATTGTTTCTCGACGTTTAATTGAAGTTGAATACACGAGCATCGCAACCACTGTAAAAAAATCATATGGTTGAAAGGTCTAAA
TAGAGTCCAACATTGAAGTCTGACTACCTATCTACATGCATATTAATAGCAAAGATGCTATACAACTTGCAGTCAATCCAGTCTATCATGAGATAACTAA
ACACATTGAGATTGACTGTCACTTTATTAAAGATAAAATTACATAAGGTTTAGTGTCTACAAAGTACATTCCTACA

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.947656
Expected error %
0.2918
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g23160.1 Score 122 E Value 2e-28 Identity 11.80% Align. Length 542bp (80.18%) Frame 3
68417.m03342 protein kinase family protein contains Pfam domain PF00069: Protein kinase domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAU89728.1| Score 195 E Value 7e-49 Identity 16.40% Align. Length 596bp (88.17%) Frame 3
putative retroelement pol polyprotein-like [Solanum tuberosum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAD19784.1| Score 182 E Value 7e-45 Identity 15.30% Align. Length 587bp (86.83%) Frame 3
putative retroelement pol polyprotein [Arabidopsis thaliana] pir||C84512 probable retroelement pol polyprotein [imported] - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0031J18
Clone
Clone name
hba-31j18
LE_HBa0031J18
C03HBa0031J18
P031J18
Clone type
BAC
Contamination
none
Estimated length
98,918
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found