Clone Read LE_HBa0077B13_T7_18121

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0077B13_T7_18121
Primer
T7
ID from Seq. Facility
LE_HBa_077_B13-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
735 trimmed for vector and quality, 935 raw
Genbank Accession
DU035756
Sequence
>LE_HBa0077B13_T7_18121 (raw untrimmed)
TATAACCGGGTTCCGTGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTAGCCCTAGGTTCATTGGACCTTTTGAGATTTTGATCCGAGTGGGAGA
GGTGGCCTATAAGTTGGCCTTGCCACCTAGTTTGTCGGCAGTTCATCCTGTTTTCCATGTCTCTATGCTTCGAAAGTATATTCCGGATGAATCTCATGTG
ATTTCACTTGATTCTGTGGAGTTGGGTCCAGACTTGACATTTGAAGAGGAGCCTATAGCTATTTTGGATAGGCAGGTTCGAAAGCTTAGAACCAAGGAGA
TTGCTTCAGTGAAGGTGCAGTGGAAGCACCGATCAGTGAGAGAGGCAATTTGGGAGACTGAGTCTGACATGCGTGCCAGATATCCTCAACTTTTTGAGGC
TTCAGGTAATTACTTTTACTCTATGTTCGAGGACGAACATGATTTTTAGTGGTGGATAATGTAATGACCCGGAAGGTCATTTTTGGAAATTTTGAATATT
TGCTTAACTTGAGTTAATTAATCGATAGTTTATGGGCTAAAGTGATAATTTATTTAAGACCCTATACTAGTTAGCCTTTATTTAATAAAATATGTGGGTA
AAATCTATCACAATTAGTACTTAATAAATTTAAATAAGGAAAAGAAAAAAAAAGGGGCGAAGGGTTTCTGTGCGGCTTACGAACTGCTAGGTAAGTTTTC
TCCTTTAATCTTCTTGATTCTGCACATAGATGTGTGCATATAATAATATTATATAATAAATTATATATTTGTGTGTGCATAATTTATTTAATGAATGAGC
TTTTATGTTTTGTGCCAATGGTGAATCAAGTTGAGTTTTTGATGTTTGTTGCTGTTTTGCCCGCTGTCCGCCCCTGGTTTTAATACCATGGGCTTCCCTT
CAAAAAAAAATAAAAAAATATTAAAATTTGTTTCA
>LE_HBa0077B13_T7_18121 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTAGCCCTAGGTTCATTGGACCTTTTGAGATTTTGATCCGAGTGGGAGAGGTGGCCTATAAGTTGGCCTTG
CCACCTAGTTTGTCGGCAGTTCATCCTGTTTTCCATGTCTCTATGCTTCGAAAGTATATTCCGGATGAATCTCATGTGATTTCACTTGATTCTGTGGAGT
TGGGTCCAGACTTGACATTTGAAGAGGAGCCTATAGCTATTTTGGATAGGCAGGTTCGAAAGCTTAGAACCAAGGAGATTGCTTCAGTGAAGGTGCAGTG
GAAGCACCGATCAGTGAGAGAGGCAATTTGGGAGACTGAGTCTGACATGCGTGCCAGATATCCTCAACTTTTTGAGGCTTCAGGTAATTACTTTTACTCT
ATGTTCGAGGACGAACATGATTTTTAGTGGTGGATAATGTAATGACCCGGAAGGTCATTTTTGGAAATTTTGAATATTTGCTTAACTTGAGTTAATTAAT
CGATAGTTTATGGGCTAAAGTGATAATTTATTTAAGACCCTATACTAGTTAGCCTTTATTTAATAAAATATGTGGGTAAAATCTATCACAATTAGTACTT
AATAAATTTAAATAAGGAAAAGAAAAAAAAAGGGGCGAAGGGTTTCTGTGCGGCTTACGAACTGCTAGGTAAGTTTTCTCCTTTAATCTTCTTGATTCTG
CACATAGATGTGTGCATATAATAATATTATATAAT

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.961652
Expected error %
0.321267
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13871.1:182-319 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.22%) Frame 2
pT7T3D cloning vector
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13869.1:118-318 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.22%) Frame 2
pT7T318U cloning vector
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAT66771.1| Score 173 E Value 5e-42 Identity 22.80% Align. Length 359bp (48.84%) Frame 3
putative polyprotein [Solanum demissum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAV31169.1| Score 171 E Value 2e-41 Identity 22.80% Align. Length 359bp (48.84%) Frame 3
putative polyprotein [Solanum tuberosum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0077B13
Clone
Clone name
hba-77b13
LE_HBa0077B13
P077B13
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found