Clone Read LE_HBa0183M06_SP6_277617

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0183M06_SP6_277617
Primer
SP6
ID from Seq. Facility
LE_HBa_183_M06-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
570 trimmed for vector and quality, 1000 raw
Sequence
>LE_HBa0183M06_SP6_277617 (raw untrimmed)
NNCCACTCTAAATCCTTGTGAGGATTCTCTTCGCCGATCAATTATGTGTCTCCAGTGCTTTTTGTCTCGTCTGTAAAATGAAGAACCCAAAAGTTTAAGA
GCTAACGGGAGTCCACCAGCATGTTTCACCACTTCTCTCGAAAGTTCAATAAAATCTTCTTTTGGTGTTTTTTCTCTAAAAGCATGCCTACTGAACAATT
CAAGTGCTTCAACCTCGGATAATAGTTGGACTTCATACACGTTATCCCCGACGTGACTGATCAACAGGTTCTTGTCTCTTGATTTAAAATAATTCTGCTA
CCCATTCCAAACCACTCTGTCCCTCCAACTAAAAATTCCAACTGTTCTCGATGGTTTACATCATCGAGAATGAACAAAACTTTCTTCCAGCGAAGCCTAT
TCTTTAAAATAATCATCCCTTCATGTTCACTAGTTACACTCATCTTTTCACCCAAGAGCTTGCGAATGACGACTTGTGCCAGCCATGCTTGTCCATTTTT
CTGGTGAAGTTCTCCAACATCACCAAGAAAACAATCAGCTTCAAATTAGATGGCGGTATCTTTCATACAGAACACGTGCAAGTTCTGATTTGCCGATGCC
ACCCATGCCACTAATACCAATAGAGCGAACATCATTTTGATTCCATACTTAATAATTCGATTACTTCCCTCAACCGGAGATCTCATAACCCCTAAGCTTT
CCGGAAAAGGGTGAAATATTTTCAATGGCCTTAAGAAAATTTTTCATCAACAAGTTTTCTGGAACCTACTCACCCTTTTTCTTTTCAATATTAATGGAAA
AATATTCCTGACAAGTAAATTTAATGGTCTCCAAAGGGATTGTTATATAGAATTAATTTGAAAATTTTACTGTGTTTAAATTTATTAGATAACACCTTTA
ATTTGCTCATTTTATGTGTTTTCTTATAAAAAATTACGTCAAATGCTGTCTGTGAAATTGTATCTTAATAAAATTGTTCTCTTATTATCCTCAAAGTTTA
>LE_HBa0183M06_SP6_277617 (trimmed for quality and cloning vector)
ATTCTCTTCGCCGATCAATTATGTGTCTCCAGTGCTTTTTGTCTCGTCTGTAAAATGAAGAACCCAAAAGTTTAAGAGCTAACGGGAGTCCACCAGCATG
TTTCACCACTTCTCTCGAAAGTTCAATAAAATCTTCTTTTGGTGTTTTTTCTCTAAAAGCATGCCTACTGAACAATTCAAGTGCTTCAACCTCGGATAAT
AGTTGGACTTCATACACGTTATCCCCGACGTGACTGATCAACAGGTTCTTGTCTCTTGATTTAAAATAATTCTGCTACCCATTCCAAACCACTCTGTCCC
TCCAACTAAAAATTCCAACTGTTCTCGATGGTTTACATCATCGAGAATGAACAAAACTTTCTTCCAGCGAAGCCTATTCTTTAAAATAATCATCCCTTCA
TGTTCACTAGTTACACTCATCTTTTCACCCAAGAGCTTGCGAATGACGACTTGTGCCAGCCATGCTTGTCCATTTTTCTGGTGAAGTTCTCCAACATCAC
CAAGAAAACAATCAGCTTCAAATTAGATGGCGGTATCTTTCATACAGAACACGTGCAAGTTCTGATTTGC

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.961149
Expected error %
0.0978488
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g40060.1 Score 63 E Value 4e-18 Identity 39.50% Align. Length 472bp (82.81%) Frame 3
68418.m04860 disease resistance protein (TIR-NBS-LRR class), putative domain signature TIR-NBS-LRR exists, suggestive of a disease resistance protein. False intron created at intron 2 to escape a frameshift in the BAC sequence.
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g72890.1 Score 62 E Value 1e-14 Identity 37.00% Align. Length 376bp (65.96%) Frame 3
68414.m08431 disease resistance protein (TIR-NBS class), putative domain signature TIR-NBS exists, suggestive of a disease resistance protein.
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA08798.1| Score 79 E Value 6e-23 Identity 36.00% Align. Length 505bp (88.60%) Frame 3
NL27 [Solanum tuberosum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAD56816.1| Score 75 E Value 7e-22 Identity 47.10% Align. Length 511bp (89.65%) Frame 3
putative resistance gene analogue protein [Lens culinaris]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0183M06
Clone
Clone name
hba-183m6
LE_HBa0183M06
C12HBa0183M06
P183M06
Clone type
BAC
Contamination
none
Estimated length
103,857
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found