Clone Read LE_HBa0103L19_T7_33205

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0103L19_T7_33205
Primer
T7
ID from Seq. Facility
LE_HBa_103_L19-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
691 trimmed for vector and quality, 794 raw
Sequence
>LE_HBa0103L19_T7_33205 (raw untrimmed)
CCCGGGGCCCGGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTATCAACTTCCAAGCAGTCTGGGTATTTTTGCTAACCGCAGGATAAATAAAG
GTAACTAAACACTGTGACAAGAGACTACTACCTAAATGAACATATTAATAATGAAATAACTTAAACAGGAACAAAGGCAACAATTAAAAATAAGATGAAA
GAAACAAATAAAGAAAGGGAATTAAGTGTTCCAACCTGGTTGTATTTTGCCAGCCGCTCACTCCGGCAGGGTGCTCCAGTCTTTATCTGCATTGGGAGAA
TTTAAAAGAATATTAATACGCTGCTGGTGGTTAAAAGGGTGGAAACGATTAACCAATGTTAACAGCTGGAAGTCTAGAGTACCTGTCCACTGGCCAAACC
AACAGACAGATCTGCGATAAAATTATCCTCTGTTTCACCACTTCGGTGACTGACCATAACACCCCATCCTGCAGCTTTGGAGTCTAGTGCTGCTTGAATA
GATTCTGTGACAGTACCGATCTGGTTAACCTGCAACAGTTTGAAAGTTGATGAGCCAAGCCCTCACTACATGACTACCATGTGTCATTCATTTACCAAGA
GAGTTCTACTGGGCACGGGCTTCCAACCCCTAATCTTATAGGAGGGATTTTATGCACATCCATGCAATGTTCCTAATCTACAGGACTGGACCCGTGATAA
TGTATGAATTGCTTTTTTGATATCTAAACCTTTTTCCCTTCTTTCCCCATCAAACTTGAAATTAAATTTAACTTTACCTTGGATAACAATCTTA
>LE_HBa0103L19_T7_33205 (trimmed for quality and cloning vector)
AGCTTATCAACTTCCAAGCAGTCTGGGTATTTTTGCTAACCGCAGGATAAATAAAGGTAACTAAACACTGTGACAAGAGACTACTACCTAAATGAACATA
TTAATAATGAAATAACTTAAACAGGAACAAAGGCAACAATTAAAAATAAGATGAAAGAAACAAATAAAGAAAGGGAATTAAGTGTTCCAACCTGGTTGTA
TTTTGCCAGCCGCTCACTCCGGCAGGGTGCTCCAGTCTTTATCTGCATTGGGAGAATTTAAAAGAATATTAATACGCTGCTGGTGGTTAAAAGGGTGGAA
ACGATTAACCAATGTTAACAGCTGGAAGTCTAGAGTACCTGTCCACTGGCCAAACCAACAGACAGATCTGCGATAAAATTATCCTCTGTTTCACCACTTC
GGTGACTGACCATAACACCCCATCCTGCAGCTTTGGAGTCTAGTGCTGCTTGAATAGATTCTGTGACAGTACCGATCTGGTTAACCTGCAACAGTTTGAA
AGTTGATGAGCCAAGCCCTCACTACATGACTACCATGTGTCATTCATTTACCAAGAGAGTTCTACTGGGCACGGGCTTCCAACCCCTAATCTTATAGGAG
GGATTTTATGCACATCCATGCAATGTTCCTAATCTACAGGACTGGACCCGTGATAATGTATGAATTGCTTTTTTGATATCTAAACCTTTTT

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.97214
Expected error %
0.757961
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g74030.1 Score 119 E Value 2e-27 Identity 21.90% Align. Length 305bp (44.14%) Frame 1
68414.m08573 enolase, putative similar to Swiss-Prot:P15007 enolase (EC 4.2.1.11) (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate hydro-lyase) [Drosophila melanogaster]
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g36530.1 Score 100 E Value 6e-22 Identity 18.60% Align. Length 305bp (44.14%) Frame 1
68415.m04481 enolase identical to SWISS-PROT:P25696 enolase (EC 4.2.1.11) (2-phosphoglycerate dehydratase)(2-phospho-D- glycerate hydro-lyase) [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAL33814.1| Score 119 E Value 9e-26 Identity 21.90% Align. Length 305bp (44.14%) Frame 1
putative enolase [Arabidopsis thaliana] gb|AAK59483.1| putative enolase [Arabidopsis thaliana] ref|NP_177543.1| enolase, putative [Arabidopsis thaliana] gb|AAG52510.1| putative enolase; 31277-33713 [Arabidopsis thaliana] pir||B96768 protein enolase F2P9.10 [imported] - Arabidopsis thaliana
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAF90638.1| Score 110 E Value 3e-23 Identity 19.90% Align. Length 305bp (44.14%) Frame 1
unnamed protein product [Tetraodon nigroviridis]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0103L19
Clone
Clone name
hba-103l19
LE_HBa0103L19
C03HBa0103L19
P103L19
Clone type
BAC
Contamination
none
Estimated length
71,738
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found