Clone Read LE_HBa0111N17_SP6_36368

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0111N17_SP6_36368
Primer
SP6
ID from Seq. Facility
LE_HBa_111_N17-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
471 trimmed for vector and quality, 811 raw
Sequence
>LE_HBa0111N17_SP6_36368 (raw untrimmed)
ATTTTTGGGTCAACCCTTTCTTCTTATGGTATTTTCTCAGCCAAGTTGGCAACCACAGCTGCTGCTGCTTCTGTTGCTGCTAGTTACCCTTTTGCTTCTA
GGGCTTTTTTTGGGTATGCGATATACACTCTTCAGTCTTTACAATGTGTTATGTTAGTTGCTATGTTTTCTTATTCTTAGTACTTGGATTTGCTACATGA
CCATGAGTTAGTGATAAGGCATACACTATGGGGATGGTGAGAGGAGATATATTTTACATCCCAGAGGGGCTATAATTGGAGATACCATTCTTTCTGGTAC
AGAAGTTCCTATAAAAATGGGAAATGCCCTACCTTTGAGTGCGGTTTGAACTATTGATTTACGTAATTTGAAATAAAAAATTTTGTTTACGACGAAACCT
AGAAATCGATCACTGATCCAATTTGAGTACCTCTGCATGATAGACTTCTATCCTCTTCAGACTCCACTTAGTGGGATTCTATTGGTTATGTTGATGTTGC
TGTTGCTGCTTATACTTCATGCTGTTATTGTATGTGAATTACTATACTTTTTAGAGGAGTGACACTTTTTCGTATTTTGTGTTTTTTTCTCAGCTTTTTA
GTCTTTTTTTGGTTCTTGGTCTAGTGTAACTATTTCCCAAATTCTTTTTCACTTTCTTAATCATTTTTTTTTTGTTCTTTTTATACTTTTAATTATTATT
TTTTACTGATTTTTTTTTTTCTACATTTTGCTTCCCCTTAACTTTAGATGTCTTTTGTTTTTTATTTTCTTATTGTACTCACATTTCTCTCTACTTCTGT
ATCGTAATTTC
>LE_HBa0111N17_SP6_36368 (trimmed for quality and cloning vector)
TTCTTATGGTATTTTCTCAGCCAAGTTGGCAACCACAGCTGCTGCTGCTTCTGTTGCTGCTAGTTACCCTTTTGCTTCTAGGGCTTTTTTTGGGTATGCG
ATATACACTCTTCAGTCTTTACAATGTGTTATGTTAGTTGCTATGTTTTCTTATTCTTAGTACTTGGATTTGCTACATGACCATGAGTTAGTGATAAGGC
ATACACTATGGGGATGGTGAGAGGAGATATATTTTACATCCCAGAGGGGCTATAATTGGAGATACCATTCTTTCTGGTACAGAAGTTCCTATAAAAATGG
GAAATGCCCTACCTTTGAGTGCGGTTTGAACTATTGATTTACGTAATTTGAAATAAAAAATTTTGTTTACGACGAAACCTAGAAATCGATCACTGATCCA
ATTTGAGTACCTCTGCATGATAGACTTCTATCCTCTTCAGACTCCACTTAGTGGGATTCTATTGGTTATGT

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.956563
Expected error %
0.253977
Sequence Annotations 
Automatic Annotations
Nicotiana tabacum Chloroplast (screening/organelle/nicotiana_tabacum_chloroplast)NCBI BLAST
gi|11465934|ref|NC_001879.1| Score 385 E Value 1e-109 Identity 96.50% Align. Length 225bp (47.77%) Frame 3
Nicotiana tabacum plastid, complete genome
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAD83422.1| Score 86 E Value 3e-16 Identity 31.70% Align. Length 125bp (26.54%) Frame 1
hypothetical protein [Nicotiana tabacum] ref|YP_173359.1| hypothetical protein NitaMp011 [Nicotiana tabacum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
pir||R5NT2D Score 82 E Value 5e-15 Identity 30.80% Align. Length 119bp (25.27%) Frame 1
ribosomal protein L2 - Debney's tobacco chloroplast sp|P21434|RK2_NICDE Chloroplast 50S ribosomal protein L2 emb|CAB52367.1| L2 protein [Nicotiana debneyi]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0111N17
Clone
Clone name
hba-111n17
LE_HBa0111N17
P111N17
Clone type
BAC
Contamination
none
Estimated length
102,813
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found