Clone Read LE_HBa0219N08_T7_396672

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0219N08_T7_396672
Primer
T7
ID from Seq. Facility
LE_HBa_219_N08-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
790 trimmed for vector and quality, 1009 raw
Sequence
>LE_HBa0219N08_T7_396672 (raw untrimmed)
GTCATGACGGGGATCTCTGAGTCGACTGCAGGCATGCAAGCTTGTCATTCAGGTATTTATCTGTTCACTTGGACCTGGAGATGAAATCATTGGTCCTCAT
TTAGAATCACCGAGATATACTTCACAACCTAAACACATCATACCGTACCAATAATTCACCACTAAAATTTTCCGTACAATATAATCCTAACTCATTCGTT
TTTATTAGCAAGGTAGTTGGTTTCTCAAATATGAATGTGACATCAAACCTTATTATACGAGCATGAGCTACAACTGATAACTCTTTATGTAGTTAGCATT
CACGCTTAACAATCAACATATCTGTTTTTATATACCACTAGTACCACATCATAAAAGTCCGTTACAGGAGATGTTTTTTATTTGCATTCACGCCTTTTGG
AAAGAGCCGCTAAATTAAGTTCGAGTTTAGGTGAAGGAAGTATGACTGCCTTACCAATAGTTGAAACTCAATCCGGAGATGTTTCGGCTTATATTCCTAC
TAATGTAATTTCCATTACTGATGGACAAATCTACCAAGAAGATCATATCCAAATAGGTCACACATTTCTGCCCATCAAATAGCTGTAAAATATTACCTAA
TTAGTTCTGTATCTAGCCTAAGCTATATGTATTCTTTCGTAGGTCAGGTCAATATAAAAAGTAACACACTCCATCTCTAATCAACTAACTATTGATACTA
TTATAGTAGAAATTCTAACCTTAATTGGTGCATCATTATTCCCATTACTTTTAAGCAACCCATTCATGTTATGATAACTTTACTAATTGTAGTTCTCCAA
GCTACATGTCCATTATCTTGTCATGCCCTTAATCATTTAACTTTCAAATTGTAAAGCACTCTAATTATCGTAAATACCACACAAGTACTATACATTTTTA
ATAGGGGAATAAACCTTACAAAACCCCCTCTCAATGGTGGAAAATTTAATTCTACCCCCAATCTTTTTCCGAAGAAAATAACTTCCTCCCTAAAATACAT
TCAAATTAA
>LE_HBa0219N08_T7_396672 (trimmed for quality and cloning vector)
GCAGGCATGCAAGCTTGTCATTCAGGTATTTATCTGTTCACTTGGACCTGGAGATGAAATCATTGGTCCTCATTTAGAATCACCGAGATATACTTCACAA
CCTAAACACATCATACCGTACCAATAATTCACCACTAAAATTTTCCGTACAATATAATCCTAACTCATTCGTTTTTATTAGCAAGGTAGTTGGTTTCTCA
AATATGAATGTGACATCAAACCTTATTATACGAGCATGAGCTACAACTGATAACTCTTTATGTAGTTAGCATTCACGCTTAACAATCAACATATCTGTTT
TTATATACCACTAGTACCACATCATAAAAGTCCGTTACAGGAGATGTTTTTTATTTGCATTCACGCCTTTTGGAAAGAGCCGCTAAATTAAGTTCGAGTT
TAGGTGAAGGAAGTATGACTGCCTTACCAATAGTTGAAACTCAATCCGGAGATGTTTCGGCTTATATTCCTACTAATGTAATTTCCATTACTGATGGACA
AATCTACCAAGAAGATCATATCCAAATAGGTCACACATTTCTGCCCATCAAATAGCTGTAAAATATTACCTAATTAGTTCTGTATCTAGCCTAAGCTATA
TGTATTCTTTCGTAGGTCAGGTCAATATAAAAAGTAACACACTCCATCTCTAATCAACTAACTATTGATACTATTATAGTAGAAATTCTAACCTTAATTG
GTGCATCATTATTCCCATTACTTTTAAGCAACCCATTCATGTTATGATAACTTTACTAATTGTAGTTCTCCAAGCTACATGTCCATTATC

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.952027
Expected error %
0.275483
Sequence Annotations 
Automatic Annotations
Nicotiana tabacum Chloroplast (screening/organelle/nicotiana_tabacum_chloroplast)NCBI BLAST
gi|11465934|ref|NC_001879.1| Score 299 E Value 7e-83 Identity 97.60% Align. Length 166bp (21.01%) Frame 1
Nicotiana tabacum plastid, complete genome
Arabidopsis thaliana Mitochondria (screening/organelle/ATH1_mitochondria)NCBI BLAST
mitochondria Score 64 E Value 2e-11 Identity 86.10% Align. Length 71bp (8.99%) Frame 3
No sequence description found.
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0219N08
Clone
Clone name
hba-219n8
LE_HBa0219N08
C08HBa0219N08
P219N08
Clone type
BAC
Contamination
none
Estimated length
45,438
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found