Clone Read LE_HBa0059H01_SP6_46800

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0059H01_SP6_46800
Primer
SP6
ID from Seq. Facility
LE_HBa_059_H01-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
682 trimmed for vector and quality, 805 raw
Sequence
>LE_HBa0059H01_SP6_46800 (raw untrimmed)
GTTTATTACCCATTTAATTTTTATNNGTTATACTTTGTAAGAGATCTTTCTATTGGATTTGAATCACTGTACTTAATTACATCTCCAAAATTAACATTGC
AGGTTAGAGCAGTCCACTCTGGAACTGCTACTCTCAAGGATGCTACTTCAGAAGCTATAAGGGATTGGGTTACAAATGTTGAAACAACTCACTACATACT
TGGATCTGTTGCAGGACCACACCCTTACCCCATGATGGTTAGGGAGTTCCATGCAGTGATTGGTAAAGAAACAAGGAAGCAATCATTGGAAAAGTGGGGC
GGGAAACCAGACGTGCTTGTTGCATGTGTTGGTGGAGGTTCAAATGCTATGGGACTCTTTCATGAGTTTGTTGATGACAAAGATGTGAGGTTGATTGGGG
TTGAAGCAGCAGGTTTTGGTCTAGATAGTAGTAAACATGCCGCCACTTTGACAAAGGGTGAGGTAGGAGTACTGCATGGAGCTATGAGTTATCTACTGCA
AGACGAAGATGGGCAGATTGTTGAGCCTCATTCTATCAGTGCCGGGTAATAACTTGAACCTTGCTTGTTACTAATGTGCTCATTTTGTGTGCTACTACAA
AATCCTATGCATACACTTGACGCTACTGAAATAAAGTGTATTTGGTCAAATCAAATCATTGTATCTCTTAACTGGTTTTGTTCCCCCAATGGATTTATTA
GATTGGATTACCCAGGAGTTGGGCCAGAACACAGCTTTTTGAAAGATCTCGGTAGAGCAGAATATTATAGCATCACAGATGAGGAGGGCTTTGGAAGGTG
ATAAT
>LE_HBa0059H01_SP6_46800 (trimmed for quality and cloning vector)
TATACTTTGTAAGAGATCTTTCTATTGGATTTGAATCACTGTACTTAATTACATCTCCAAAATTAACATTGCAGGTTAGAGCAGTCCACTCTGGAACTGC
TACTCTCAAGGATGCTACTTCAGAAGCTATAAGGGATTGGGTTACAAATGTTGAAACAACTCACTACATACTTGGATCTGTTGCAGGACCACACCCTTAC
CCCATGATGGTTAGGGAGTTCCATGCAGTGATTGGTAAAGAAACAAGGAAGCAATCATTGGAAAAGTGGGGCGGGAAACCAGACGTGCTTGTTGCATGTG
TTGGTGGAGGTTCAAATGCTATGGGACTCTTTCATGAGTTTGTTGATGACAAAGATGTGAGGTTGATTGGGGTTGAAGCAGCAGGTTTTGGTCTAGATAG
TAGTAAACATGCCGCCACTTTGACAAAGGGTGAGGTAGGAGTACTGCATGGAGCTATGAGTTATCTACTGCAAGACGAAGATGGGCAGATTGTTGAGCCT
CATTCTATCAGTGCCGGGTAATAACTTGAACCTTGCTTGTTACTAATGTGCTCATTTTGTGTGCTACTACAAAATCCTATGCATACACTTGACGCTACTG
AAATAAAGTGTATTTGGTCAAATCAAATCATTGTATCTCTTAACTGGTTTTGTTCCCCCAATGGATTTATTAGATTGGATTA

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.963412
Expected error %
0.0215852
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g27070.1 Score 289 E Value 9e-79 Identity 30.30% Align. Length 455bp (66.72%) Frame 1
68417.m03892 tryptophan synthase, beta subunit 2 (TSB2) identical to SP|25269
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g54810.1 Score 288 E Value 2e-78 Identity 30.30% Align. Length 455bp (66.72%) Frame 1
68418.m06827 tryptophan synthase, beta subunit 1 (TSB1) identical to SP|P14671
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAD83779.1| Score 298 E Value 1e-79 Identity 29.80% Align. Length 485bp (71.11%) Frame 1
tryptophan synthase beta subunit [Polygonum tinctorium]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAB97526.1| Score 296 E Value 3e-79 Identity 31.40% Align. Length 455bp (66.72%) Frame 1
tryptophan synthase beta [Camptotheca acuminata] gb|AAB97087.1| tryptophan synthase beta subunit [Camptotheca acuminata] sp|O50046|TRPB_CAMAC Tryptophan synthase beta chain 2, chloroplast precursor
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0059H01
Clone
Clone name
hba-59h1
LE_HBa0059H01
P059H01
Clone type
BAC
Contamination
none
Estimated length
136,653
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found