Clone Read LE_HBa0011E22_T7_5493

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0011E22_T7_5493
Primer
T7
ID from Seq. Facility
LE_HBa_011_E22-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
833 trimmed for vector and quality, 934 raw
Sequence
>LE_HBa0011E22_T7_5493 (raw untrimmed)
CGTAACCTGGTACCGAGGGTTCCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGAAAGGTGGTGAAATGATGGTCATTAAGAGGAAAACTGAAGATTTTT
CTAGTGGATCTACTACCGAGGCAAACCATTTAGCCGGGTTGGGGGTTGGTGAAGTCGGTATTCGCGAAGGAAGAATGCTCTTCATCTCCCCTCGTTATAA
TTATCTCTGGAAGTCGCCCCTACAACCCCTTGACTCCTGCAGGCTACGTTCGAACGAAACGAGAGTAAGGCTTTCTCCACGAGAGAACAGACTGACTACT
TGGAGAGGGGCTATTCACGGAACGTGAGAAGAAGATGATTAATCATCTGTTTCCGGAAGAAATCGAAGAATTTCTTGGGAATCCTAGGTGGAAACAAAAA
TAAACCTCAGGAAGTCTCTGAGAATTGCTAGTATGCAAATAAACGTCTACGAACGATAAAGTAACAATGAATCTATTAGATAAAAACTTACCAAATATTT
TCATCAACTCGTGGAATTAAATAGAAAGAAAACTATAATATCATCATCATTATTGGTAATTGATTTAATTTAATTTCAACATGTGATATAAAATTCTTTG
AAAATTGAAAAAGATTTAAAAACTATCACAGTGTAATTGGAATGAACTCAGAGACCACCGTATATAGTTAGATTAAAAGTTGAGTGATCAAGTAGAAAGC
TGGAAAGGACAGAAATAAATAGCAAATTAGATATAATTTATACTGATAATGGTATATGTACTTCTAGTAACGGTTGACAAAATGATACAAAGAGATCTCA
TTGAATTTGTGAATAAAATGAGGGGGGCATGGGGGATTTACCATAGGAGTCTTTTATTATTTATTCAATACTAAGCTTCCTTTCCGTTTTATTCTATATC
TTTTTTCCTTTAGTCATTAAAGTTGTTACTCTAC
>LE_HBa0011E22_T7_5493 (trimmed for quality and cloning vector)
AGCTTGAAAGGTGGTGAAATGATGGTCATTAAGAGGAAAACTGAAGATTTTTCTAGTGGATCTACTACCGAGGCAAACCATTTAGCCGGGTTGGGGGTTG
GTGAAGTCGGTATTCGCGAAGGAAGAATGCTCTTCATCTCCCCTCGTTATAATTATCTCTGGAAGTCGCCCCTACAACCCCTTGACTCCTGCAGGCTACG
TTCGAACGAAACGAGAGTAAGGCTTTCTCCACGAGAGAACAGACTGACTACTTGGAGAGGGGCTATTCACGGAACGTGAGAAGAAGATGATTAATCATCT
GTTTCCGGAAGAAATCGAAGAATTTCTTGGGAATCCTAGGTGGAAACAAAAATAAACCTCAGGAAGTCTCTGAGAATTGCTAGTATGCAAATAAACGTCT
ACGAACGATAAAGTAACAATGAATCTATTAGATAAAAACTTACCAAATATTTTCATCAACTCGTGGAATTAAATAGAAAGAAAACTATAATATCATCATC
ATTATTGGTAATTGATTTAATTTAATTTCAACATGTGATATAAAATTCTTTGAAAATTGAAAAAGATTTAAAAACTATCACAGTGTAATTGGAATGAACT
CAGAGACCACCGTATATAGTTAGATTAAAAGTTGAGTGATCAAGTAGAAAGCTGGAAAGGACAGAAATAAATAGCAAATTAGATATAATTTATACTGATA
ATGGTATATGTACTTCTAGTAACGGTTGACAAAATGATACAAAGAGATCTCATTGAATTTGTGAATAAAATGAGGGGGGCATGGGGGATTTACCATAGGA
GTCTTTTATTATTTATTCAATACTAAGCTTCCT

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.956377
Expected error %
0.736241
Sequence Annotations 
Automatic Annotations
Nicotiana tabacum Chloroplast (screening/organelle/nicotiana_tabacum_chloroplast)NCBI BLAST
gi|11465934|ref|NC_001879.1| Score 147 E Value 6e-37 Identity 98.70% Align. Length 77bp (9.24%) Frame 1
Nicotiana tabacum plastid, complete genome
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0011E22
Clone
Clone name
hba-11e22
LE_HBa0011E22
P011E22
Clone type
BAC
Contamination
none
Estimated length
99,099
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found