Clone Read LE_HBa0012O12_SP6_6454

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0012O12_SP6_6454
Primer
SP6
ID from Seq. Facility
LE_HBa_012_O12-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
647 trimmed for vector and quality, 891 raw
Genbank Accession
DU025907
Sequence
>LE_HBa0012O12_SP6_6454 (raw untrimmed)
TTAAACTACCTTACCGTACCCACTATGGTACTATTCATAAATACAGTTTCCTTATTTTATCAAAAACTAATTAGTATGTTACAAAATAATCCCTTATATT
ACCCTTCTGCAGGTCAAGAAACATATCAAACAAGGGCAAGGTCATGAAGGTGGAATATTTACAGTTGAAGCCCCGCTGCATGTTTCCAATGTTCAGGTTG
TAGATCCAGTCACAGGGTATGGATATATTATTTCTCTTTACCTTTTATTTTTTGCTCTTAGAACATGGTAGCTCATGTCTTCTTCCACAGAAAACCAACC
AAGGTCGGAATTAGATACCTGGAGGATGGCTCCAAAGTAAGAGTCTCAAGAGGTATAGGAGCATCTGGCTCTATCATTCCCCGGCCTGAGATCTTAAAAA
TAAGGACCACACCAAGGCCTACAGTTGGTAATTATCAAATCCTTTTACGTATGAGTATGTTCTTCTAAATGAGTATTCTGTTGCATTTCAAATGTAGTAA
CTGCTGTATCTTTTTGTATCTTTTTTTTTTTGTGGCGGGAGGTTACTTAAAAAAATAAGTTTATTGGTTTAGAGGTATAATCTGATTTCAACTGGAAGTA
GAAATGAAAGGTTAAAACTTTAGAGAGCTTCGGATCAATAGTAGTCAACTGCATGATGCCACCTTAATGTGAGACAAGTTTTCATTGAAAACAAATTGTT
GAAATATACATGAAGAGATTATGGTTGGGTTGATAAAGTATAAATTTGAAATTAGAATTTTAAGTCCAAATTTTAGATGAACATCCATTCTGGGAGAGCT
CCAAAAAACTGGGTTTTATTTCTTCCATTTCCCTCCCCTTGGGTTTCCAAAAAGGGGGGAAATACGGTTTGGGGAAAAAATGGAAAGGCCC
>LE_HBa0012O12_SP6_6454 (trimmed for quality and cloning vector)
TGGTACTATTCATAAATACAGTTTCCTTATTTTATCAAAAACTAATTAGTATGTTACAAAATAATCCCTTATATTACCCTTCTGCAGGTCAAGAAACATA
TCAAACAAGGGCAAGGTCATGAAGGTGGAATATTTACAGTTGAAGCCCCGCTGCATGTTTCCAATGTTCAGGTTGTAGATCCAGTCACAGGGTATGGATA
TATTATTTCTCTTTACCTTTTATTTTTTGCTCTTAGAACATGGTAGCTCATGTCTTCTTCCACAGAAAACCAACCAAGGTCGGAATTAGATACCTGGAGG
ATGGCTCCAAAGTAAGAGTCTCAAGAGGTATAGGAGCATCTGGCTCTATCATTCCCCGGCCTGAGATCTTAAAAATAAGGACCACACCAAGGCCTACAGT
TGGTAATTATCAAATCCTTTTACGTATGAGTATGTTCTTCTAAATGAGTATTCTGTTGCATTTCAAATGTAGTAACTGCTGTATCTTTTTGTATCTTTTT
TTTTTTGTGGCGGGAGGTTACTTAAAAAAATAAGTTTATTGGTTTAGAGGTATAATCTGATTTCAACTGGAAGTAGAAATGAAAGGTTAAAACTTTAGAG
AGCTTCGGATCAATAGTAGTCAACTGCATGATGCCACCTTAATGTGA

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.958927
Expected error %
0.231023
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g23535.1 Score 77 E Value 2e-28 Identity 79.50% Align. Length 310bp (47.91%) Frame 2
68418.m02762 KOW domain-containing protein simlar to SP|P12876 50S ribosomal protein L24 (BL23) (12 kDa DNA-binding protein) (HPB12) {Bacillus subtilis}; contains Pfam profile PF00467: KOW motif
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAA97247.1| Score 77 E Value 8e-27 Identity 79.50% Align. Length 310bp (47.91%) Frame 2
50S ribosomal protein L24 [Arabidopsis thaliana] ref|NP_680212.1| KOW domain-containing protein [Arabidopsis thaliana] gb|AAS76726.1| At5g23535 [Arabidopsis thaliana] gb|AAS47607.1| At5g23535 [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0012O12
Clone
Clone name
hba-12o12
LE_HBa0012O12
C08HBa0012O12
P012O12
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found