Clone Read LE_HBa0105L22_T7_68539

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0105L22_T7_68539
Primer
T7
ID from Seq. Facility
LE_HBa_105_L22-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
697 trimmed for vector and quality, 919 raw
Sequence
>LE_HBa0105L22_T7_68539 (raw untrimmed)
CTAAGTGGGGCCCGTGGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTCTTTCATTTAAACGTCCACACCTGTAAAGAGCTAGTTCTTTTTTCCAT
TACTGGGAGAAGTACCCCAAGCTACATTTCTAGTTTACAGGAGAAAAGATCTGCAACAGGCAGATTTCAGATGTACAAAGCACAAGCATAGTACAAAACA
GGCCCGATCAGATGAAACTTTCCGGCTCATTGAAAGAGTCAGAATTGAGGTATAATTTCAAAGGATTTAAAATTTTGTCCAACAAAGCTTCAAAAAAACT
GTGGTTGTTGTGACCGTAACCTGCTCTTCATCCAGCAAAGATAAAAATGCACATTCCATTGACACAGGAGGACTTACGCCAAAAGAACCAAGCTGGATGA
CTAAGTCACATTCATGTGCAATGTTCAATTGAAACTGGTGTTCATGCGTCAGCGGCTCTAGCCCAAATATTGACATCAGCATATCATGCTTCTTAACATG
ATTAATTTATAATTATCCAACTCCTAGAATTTATGGAGTTTTTATTTATTTCCTTGCAAGTCATTTCTTGATTGAGAAGACCAAATCTTCGTAGCTAAAA
ATGGGATTTTAGAGTCTCCTTTTCATAAATAAATCTATCAGATCCATCTGTGTAGCTATAAATAACTTCTAATCATCAATGAAAAATTTTTGCCTCTAAC
AGTGAAACCAAAAAAAAAAACCAGACCTCACTTGTGGGATTTACTGGCTATGTTGTCGTTAGTTTTTCCCTCAAACTTTTCAGAATAGCTAAGGCAAGTC
CACTTCTAAGGTTCTCTCATTATTTCCCCGGTAAGACTAACCTTTGACTGATCAATGAAAATTAATTATTAACTCCCACCATCCCCTAAGCTCGGAAAGG
CTTCTATATTTAACACAAG
>LE_HBa0105L22_T7_68539 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTCTTTCATTTAAACGTCCACACCTGTAAAGAGCTAGTTCTTTTTTCCATTACTGGGAGAAGTACCCCAAG
CTACATTTCTAGTTTACAGGAGAAAAGATCTGCAACAGGCAGATTTCAGATGTACAAAGCACAAGCATAGTACAAAACAGGCCCGATCAGATGAAACTTT
CCGGCTCATTGAAAGAGTCAGAATTGAGGTATAATTTCAAAGGATTTAAAATTTTGTCCAACAAAGCTTCAAAAAAACTGTGGTTGTTGTGACCGTAACC
TGCTCTTCATCCAGCAAAGATAAAAATGCACATTCCATTGACACAGGAGGACTTACGCCAAAAGAACCAAGCTGGATGACTAAGTCACATTCATGTGCAA
TGTTCAATTGAAACTGGTGTTCATGCGTCAGCGGCTCTAGCCCAAATATTGACATCAGCATATCATGCTTCTTAACATGATTAATTTATAATTATCCAAC
TCCTAGAATTTATGGAGTTTTTATTTATTTCCTTGCAAGTCATTTCTTGATTGAGAAGACCAAATCTTCGTAGCTAAAAATGGGATTTTAGAGTCTCCTT
TTCATAAATAAATCTATCAGATCCATCTGTGTAGCTATAAATAACTTCTAATCATCAATGAAAAATTTTTGCCTCTAACAGTGAAACCAAAAAAAAA

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.95347
Expected error %
0.267238
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|L24367.1:218-930 Score 66 E Value 5e-12 Identity 100.00% Align. Length 32bp (4.59%) Frame 2
Cloning vector pTugAS (derived from E. coli JM105) beta-lactamase and repressor protein gene, complete cds
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|X65303.1:1-108 Score 64 E Value 2e-11 Identity 100.00% Align. Length 31bp (4.45%) Frame 2
Cloning vector pGEM-4
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0105L22
Clone
Clone name
hba-105l22
LE_HBa0105L22
P105L22
Clone type
BAC
Contamination
none
Estimated length
114,050
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found