Clone Read LE_HBa0128I14_T7_69147

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0128I14_T7_69147
Primer
T7
ID from Seq. Facility
LE_HBa_128_I14-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
477 trimmed for vector and quality, 902 raw
Genbank Accession
DU074170
Sequence
>LE_HBa0128I14_T7_69147 (raw untrimmed)
TTAACTTGGGGTCCGGGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTGGTGTTTTTCCTCTTCTTCTCGATTGTTTCTCTCTCTCCCTTCTTGT
TCTTTCTATTTTCTTATTCAAACCCTCCTTCTTTTACCCTAATTAGTATATAATTAAGAATAAAAGATGGCAATAATACCCCACTAATTAACTTAGGGTT
ACCTCTTTTAACCCCCAAGAATTTGAGTTATTCATATAAACCCACGAACTTTATAATTAAGGTAAGAATAGTCAAAAACGTCCCTTAAAACTTAACCAGA
AATCCGACTCCAACTGGGATTACGCAAACTGTGACGGGCCGTCGTGCCTGCGACGGTCCGTCCTGCAGGTCGTCGCAGAGTTCAGAGACCCAAATTTCCA
CCAAGGGTCTGTGACGGTCCGTCACACCTGTGACGGTCCGTCCTGCCATTCCGTCTCAAAGTTCAGAGAGTCGATTTTCAGTACCCAATTTCAGATTTTT
CTAAGTGTTTAGAAACGAGACCCTGCGACGGTCCGTCGTGCCCATGACGTGTTCGTCGTGCGGTTCTTCTCTCTTATTCTGATTTTTTATATAAGAAATC
TAAGTGCACAGAACGAGCAAACCGGTCGATATCGTATCTTACTTTCGCTTTAGGGTTCCTGTGTGAGGGAAGTTTCCTGGTACTCCTTTCTATATATATT
CTCTTTCCTTCATCCTAGTTTTTATAACTTACATATCGCAGACTCTCCCCTTATTTGCCTTCACTAATTTGTGTATGATTGTTTCGTATCGTTGGCTTAT
CCTTAATTTGATTACCATTGTTTATAAAACTGTCTCTATTTTAAATTCTTCGATAATTGATTTTAATCTATTTAATTTATATATAATTATTTCTAATGTT
TA
>LE_HBa0128I14_T7_69147 (trimmed for quality and cloning vector)
CTAGAGTCGACCTGCAGGCATGCAAGCTTGGTGTTTTTCCTCTTCTTCTCGATTGTTTCTCTCTCTCCCTTCTTGTTCTTTCTATTTTCTTATTCAAACC
CTCCTTCTTTTACCCTAATTAGTATATAATTAAGAATAAAAGATGGCAATAATACCCCACTAATTAACTTAGGGTTACCTCTTTTAACCCCCAAGAATTT
GAGTTATTCATATAAACCCACGAACTTTATAATTAAGGTAAGAATAGTCAAAAACGTCCCTTAAAACTTAACCAGAAATCCGACTCCAACTGGGATTACG
CAAACTGTGACGGGCCGTCGTGCCTGCGACGGTCCGTCCTGCAGGTCGTCGCAGAGTTCAGAGACCCAAATTTCCACCAAGGGTCTGTGACGGTCCGTCA
CACCTGTGACGGTCCGTCCTGCCATTCCGTCTCAAAGTTCAGAGAGTCGATTTTCAGTACCCAATTTCAGATTTTTC

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.962876
Expected error %
0.257517
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U12668.1:2492-2589 Score 62 E Value 5e-11 Identity 100.00% Align. Length 30bp (6.29%) Frame 2
GUS gene fusion vector pBI101.2 T-DNA region
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U12640.1:2493-2608 Score 62 E Value 5e-11 Identity 100.00% Align. Length 30bp (6.29%) Frame 2
GUS gene fusion vector pBI101.3 T-DNA region
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0128I14
Clone
Clone name
hba-128i14
LE_HBa0128I14
P128I14
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found