Clone Read LE_HBa0033N22_SP6_7194

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0033N22_SP6_7194
Primer
SP6
ID from Seq. Facility
LE_HBa_033_N22-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
813 trimmed for vector and quality, 911 raw
Genbank Accession
DU026555
Sequence
>LE_HBa0033N22_SP6_7194 (raw untrimmed)
CGACTATTGTAAAAGAGCAGCCTGAATGGTTAAAGGGTGGGAAACTTCGAGATTATCAGCTTGAAGGCTTAAATTTTCTGGTTAATAGGTAATGCAGTTG
GCTCTGCATTATGGTTATATATGTTTTCTTCCCACGAGTTTTTGCTTTCTGATATGTCTTCTCACAGCACAGTTGGAGGAATGATACTAATGTTATATTA
GCTGATGAAATGGGCCTCGGCAAAACTGTTCAGTCTGTTTCAATGCTCGGTTTTCTTCAGGTGAGGACTTCTCTTTGTTTTCTGCTCTTTGAAGTATTGC
TATTTCAATTTTGGTAAACGGTATTATTTTTTGTCCCTGCAGAATGCTCAGCAAATTCATGGACCATTTCTTGTTGTTGTCCCATTATCAACTCTTGCCA
ATTGGGCAAAAGAATTTAGGAAATGGCTGCCTGATATGAACGTTATTGTCTATGTCGGAGCACGTGCAAGTCGTGAGGTAAGTCTGGATTATACAGTGTT
CACTGTTCATGCTGTATTTTGTTTGATTTGGTAATTATCTAGTTACTCGGTTGTTAACTTTAATGACTAGGGAAATGCTATCTGTATTCTTTTTCCAGGA
ATGTTGTATGTTGATTCAAGCACAATTGTGTGTGATTTTACTACTAGTGTCTTCATTTTTATTTTATTCGTCTGTACATTCTTAATTTACTTGATATACT
GTTGCATGTTAAGGTCTTTTATTTGTTGGGTGTGATGTTTATATAAATACGAAACAGTACTTATTCTTTTTCGGTTACTTCTCTGTACATTCTTTTTTCT
TTGTAAGCAAACACTGGTTTTGGCACTTGAGCCCCCCGCCTAACCATTTGACAACACTGTGCATACTTTGACAACACTGTGCAGTGTGCTCATGAAAATT
GGGGGTTTAAC
>LE_HBa0033N22_SP6_7194 (trimmed for quality and cloning vector)
CCTGAATGGTTAAAGGGTGGGAAACTTCGAGATTATCAGCTTGAAGGCTTAAATTTTCTGGTTAATAGGTAATGCAGTTGGCTCTGCATTATGGTTATAT
ATGTTTTCTTCCCACGAGTTTTTGCTTTCTGATATGTCTTCTCACAGCACAGTTGGAGGAATGATACTAATGTTATATTAGCTGATGAAATGGGCCTCGG
CAAAACTGTTCAGTCTGTTTCAATGCTCGGTTTTCTTCAGGTGAGGACTTCTCTTTGTTTTCTGCTCTTTGAAGTATTGCTATTTCAATTTTGGTAAACG
GTATTATTTTTTGTCCCTGCAGAATGCTCAGCAAATTCATGGACCATTTCTTGTTGTTGTCCCATTATCAACTCTTGCCAATTGGGCAAAAGAATTTAGG
AAATGGCTGCCTGATATGAACGTTATTGTCTATGTCGGAGCACGTGCAAGTCGTGAGGTAAGTCTGGATTATACAGTGTTCACTGTTCATGCTGTATTTT
GTTTGATTTGGTAATTATCTAGTTACTCGGTTGTTAACTTTAATGACTAGGGAAATGCTATCTGTATTCTTTTTCCAGGAATGTTGTATGTTGATTCAAG
CACAATTGTGTGTGATTTTACTACTAGTGTCTTCATTTTTATTTTATTCGTCTGTACATTCTTAATTTACTTGATATACTGTTGCATGTTAAGGTCTTTT
ATTTGTTGGGTGTGATGTTTATATAAATACGAAACAGTACTTATTCTTTTTCGGTTACTTCTCTGTACATTCTTTTTTCTTTGTAAGCAAACACTGGTTT
TGGCACTTGAGCC

Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.94059
Expected error %
0.051662
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g13370.1 Score 91 E Value 2e-38 Identity 61.30% Align. Length 459bp (56.46%) Frame 2
68415.m01476 chromodomain-helicase-DNA-binding family protein / CHD family protein similar to SP|O14647 Chromodomain-helicase-DNA-binding protein 2 (CHD-2) {Homo sapiens}; contains Pfam profiles PF00271: Helicase conserved C-terminal domain, PF00176: SNF2 family N-terminal domain, PF00385: 'chromo' (CHRromatin Organization MOdifier)
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At4g31900.1 Score 54 E Value 4e-17 Identity 43.00% Align. Length 480bp (59.04%) Frame 2
68417.m04533 chromatin remodeling factor, putative strong similarity to chromatin remodeling factor CHD3 (PICKLE) [Arabidopsis thaliana] GI:6478518; contains Pfam profiles PF00271: Helicase conserved C-terminal domain, PF00176: SNF2 family N-terminal domain
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
dbj|BAD94038.1| Score 93 E Value 2e-37 Identity 61.30% Align. Length 480bp (59.04%) Frame 2
pseudogene [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAD28668.1| Score 91 E Value 8e-37 Identity 61.30% Align. Length 459bp (56.46%) Frame 2
putative chromodomain-helicase-DNA-binding protein [Arabidopsis thaliana] pir||C84507 hypothetical protein At2g13370 [imported] - Arabidopsis thaliana
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0033N22
Clone
Clone name
hba-33n22
LE_HBa0033N22
P033N22
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found