Clone Read LE_HBa0154H15_T7_75245

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0154H15_T7_75245
Primer
T7
ID from Seq. Facility
LE_HBa_154_H15-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
420 trimmed for vector and quality, 968 raw
Genbank Accession
DU066036
Sequence
>LE_HBa0154H15_T7_75245 (raw untrimmed)
GCCTGACCTGGCCCGCGGGGATCTCTAGAGTCGACCTGCAGGCATGCAAGCTTATGGCAAAAGGTATGAATGAGCCTGTCTTTGTTCTAAGAGTGAGTGT
TGAAAAGTAATCCTTATACTCAAACTTAAATCATTGTGTGAAAAAGGAGGATTTTGTTTTAAAGTGAGGACACTAGTTACAATACCGAAATTGTTAGCAC
CTCGGTGAGAAATTGAAGAGGATATGTGTTAGTGCGTTGTGAGTCTGTGTCATGTTCTGATCCCACAAAATTCAAGCCTAATAGTGATAGCATGCATAGA
TGTGATGAGATTAATCGGGATTGATAGCCAAATGATTGCAAAGGATAAAACATGGATACTATTGTACAAAGTTTGTGTAGTGAGTCATAGTGCGTCGCTT
GAGGACAAACAAAGAATATAAGTTGAGGGTGTTGATATACCGTGGTTTCATAGTATTATTATTACTTTTTCCTTAACTTTAGAATTGTCCACAAGCCTTC
TTGCGCCTATGTTTAGAGAAATTGCCTCGTTATATTACACGAGTCTGTCCAGATTCTTCACGCCAGGCATATTCCATCCATTGTTTATGAATATAGAGAT
CTCCCCTCCGAGTCCTTACACTTTTCACTCTTAATATCTGGTGTGTCTAATGCTCGGTGTGCACGATGGAATTCTTCTTTTTTTTGTCTGTTTCAACTTT
TTTTTGCCTCATGTTTATTATCTTGTCTTCATGGTTCTATTGTGCTAATCAGCCGCTTCACTCATTTTATCATGTGGATTATCTTATTATATATTTTAAT
TATCGGAAAGAATAACGGAAAAAATAAATATACATCAGGAACGTTATACAATACGTTCAAAAAACAAACTCAGATCTATCATCTATTTATGATATATTCG
ATCTGACTGTGCTTTCTTAGTTTTTGTCTTCTTTTGCCCTTATTCGTTTTTCCTACTTTTTGCTGTCT
>LE_HBa0154H15_T7_75245 (trimmed for quality and cloning vector)
CTCTAGAGTCGACCTGCAGGCATGCAAGCTTATGGCAAAAGGTATGAATGAGCCTGTCTTTGTTCTAAGAGTGAGTGTTGAAAAGTAATCCTTATACTCA
AACTTAAATCATTGTGTGAAAAAGGAGGATTTTGTTTTAAAGTGAGGACACTAGTTACAATACCGAAATTGTTAGCACCTCGGTGAGAAATTGAAGAGGA
TATGTGTTAGTGCGTTGTGAGTCTGTGTCATGTTCTGATCCCACAAAATTCAAGCCTAATAGTGATAGCATGCATAGATGTGATGAGATTAATCGGGATT
GATAGCCAAATGATTGCAAAGGATAAAACATGGATACTATTGTACAAAGTTTGTGTAGTGAGTCATAGTGCGTCGCTTGAGGACAAACAAAGAATATAAG
TTGAGGGTGTTGATATACCG

This is probably not a useful sequence.

Problems:
  • Probable foreign DNA contamination
 Warnings:
  • Not screened for chimera
  • Not screened for repetitive elements
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.944502
Expected error %
0.422939
Sequence Annotations 
Automatic Annotations
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|U13869.1:118-318 Score 66 E Value 3e-12 Identity 100.00% Align. Length 32bp (7.62%) Frame 2
pT7T318U cloning vector
NCBI UniVec vector sequences (screening/vector/UniVec)NCBI BLAST
gnl|uv|L38948.1:25-452 Score 66 E Value 3e-12 Identity 100.00% Align. Length 32bp (7.62%) Frame 2
Cloning vector pKIL128 ccdB gene, complete cds
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0154H15
Clone
Clone name
hba-154h15
LE_HBa0154H15
P154H15
Clone type
BAC
Contamination
none
Estimated length
137,077
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found