Clone Read LE_HBa0157F16_SP6_75918

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0157F16_SP6_75918
Primer
SP6
ID from Seq. Facility
LE_HBa_157_F16-SP6
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
615 trimmed for vector and quality, 827 raw
Genbank Accession
DU063095
Sequence
>LE_HBa0157F16_SP6_75918 (raw untrimmed)
GATATGGTGATGAGGGAATGGGACCAATTCCATCAAGGTGGAAAGGAACTTGCCAAACTGGCTCTGATCCCACATTTCGTTGCAATAGGTATTTTCTCTT
TAATATTTAGGTAATGTTCAAAAGTTAGTTTGAGTACTTCAATGAATTTCTATTCCTTGAACGTTGCGAAAAGGAAAGTGGAACGAATGAAGTATGATAT
ATACATGTAACATGTTTATGTTAGTGTAACATCAAGATGACTTTTGACCTATGGAGTTTGACTTTAAATATTGAAGGAAGTTAATTGGAGCAAGATACTT
CATCAAAGGATTTGCTGCTGAGGCAGGAGCTCTAGTAACCTCCAAATTCTACACGCCACGAGACACGTTAGGGCATGGATCACACACGTTGTCCACAGCT
GGAGGTAATTTTGTCGAGGGAGCTAACATTTTCGGATATGGCAATGGAACAGCTAAGGGAGGGTCACCAAGAGCACGAGTTGCGTCTTATAAGGCTTGTT
GGCCTCCTATTATCCCATCCGATTCGTGTACTGATGCAGATGTCTTGGCTGCATTTGACATGGCAATTCATGATGGCGTTGACGTGCTTTCCTTATCAAT
GGGAGCGTTGCCTGTACCATATGCCCAAGACAGTATTGCTATCGGGTTCGTTTTCATGCTATAAAACGTGGCATTGTTGGCGTTACCTAAAGTGGGAATT
CAGGTGCCTATCCTGGTACAAAAGAAAAATCTTGCACTTTGGCTTATTAATTGTCCTGTAATTTTTTTTTTTTTGTTATTTTTCTTGTTTTATACCTCCT
AAGGGAAAAGAAAAAAACGATAAAAAA
>LE_HBa0157F16_SP6_75918 (trimmed for quality and cloning vector)
TGGGACCAATTCCATCAAGGTGGAAAGGAACTTGCCAAACTGGCTCTGATCCCACATTTCGTTGCAATAGGTATTTTCTCTTTAATATTTAGGTAATGTT
CAAAAGTTAGTTTGAGTACTTCAATGAATTTCTATTCCTTGAACGTTGCGAAAAGGAAAGTGGAACGAATGAAGTATGATATATACATGTAACATGTTTA
TGTTAGTGTAACATCAAGATGACTTTTGACCTATGGAGTTTGACTTTAAATATTGAAGGAAGTTAATTGGAGCAAGATACTTCATCAAAGGATTTGCTGC
TGAGGCAGGAGCTCTAGTAACCTCCAAATTCTACACGCCACGAGACACGTTAGGGCATGGATCACACACGTTGTCCACAGCTGGAGGTAATTTTGTCGAG
GGAGCTAACATTTTCGGATATGGCAATGGAACAGCTAAGGGAGGGTCACCAAGAGCACGAGTTGCGTCTTATAAGGCTTGTTGGCCTCCTATTATCCCAT
CCGATTCGTGTACTGATGCAGATGTCTTGGCTGCATTTGACATGGCAATTCATGATGGCGTTGACGTGCTTTCCTTATCAATGGGAGCGTTGCCTGTACC
ATATGCCCAAGACAG

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
flanking vector not found in read
Quality trim threshold
20.5
Sequence entropy
0.975844
Expected error %
0.621482
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g04160.1 Score 160 E Value 5e-40 Identity 22.40% Align. Length 356bp (57.89%) Frame 3
68415.m00400 subtilisin-like protease (AIR3) almost identical to subtilisin-like protease AIR3 GI:4218991 from [Arabidopsis thaliana], missing 200 aa at N-terminus
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At5g59810.1 Score 147 E Value 4e-36 Identity 20.70% Align. Length 356bp (57.89%) Frame 3
68418.m07499 subtilase family protein subtilisin-like protease AIR3, Arabidopsis thaliana, EMBL:AF098632
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAD12260.1| Score 160 E Value 2e-38 Identity 22.40% Align. Length 356bp (57.89%) Frame 3
subtilisin-like protease [Arabidopsis thaliana] ref|NP_565309.2| subtilisin-like protease (AIR3) [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAM15483.1| Score 160 E Value 2e-38 Identity 22.40% Align. Length 356bp (57.89%) Frame 3
subtilisin-like serine protease AIR3 [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0157F16
Clone
Clone name
hba-157f16
LE_HBa0157F16
P157F16
Clone type
BAC
Contamination
none
Estimated length
90,149
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found