Clone Read LE_HBa0150A22_T7_90283

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0150A22_T7_90283
Primer
T7
ID from Seq. Facility
LE_HBa_150_A22-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
560 trimmed for vector and quality, 799 raw
Genbank Accession
DU058512
Sequence
>LE_HBa0150A22_T7_90283 (raw untrimmed)
AGCCCGGTACCGGCGGGACCTCTAGAGTCGACCTGCAGGCATGCAAGCTTTTTTTACAATTCTAATGTCAACCCTTCAGAAACCGATCTACACGACTCTC
TACGAGTTCGTCACAATCCAGTAGCTAGCTTTATTCTAAAAATCCACTCAAAATCCTAACTCTACATCCACAAACAAATATCTAATACTCCCTCCTGTAC
CAATTTTAAGTGTCTTACTTTCCTTTTTGGAATGTCTCTTCCTATATTTCATTAGTTCTTCAATTGCAAAATTAAGACCCCGAGATTTAAAGGACTACAC
ACATCTTTCTAAAACTATACTTTTTGAAGCTCAATTTTGAAATACTAAAAGGGAACTGAAATCAAATCAAATTTTCAAAATAAACCCCAATTTAAGAGAG
AGAAAGTAAGTTACCATCCAATAAGGTAACCAAAAGCAATGAAGAAAGTGATGATCCCACAATAAGGCCGATAGATAACCATAGAAGCATAACCCAAAAG
CATAGGCACCAAAAAGTGAAGATTTGAACTGAATCCGAAAGACAAGTATGAAAGAACACCACCCATCACGGCGGCGTAGAGATGTCTTCCGGCGGAAGAA
GGAACAAAATGGTGGAAGAAGCTTCTCGGAATAGGTGCAAAGAAACAAATAAAGAAATCAGAGCACCGGCTCATATACTCCAATCCGCCGCTATCCATTA
ATGTTCTTCTCGGGCCGCATTCCTATTAGGAAAAACAAATAACTTGGCTTCGAGATTTATATAAATTTTTCCGCCTTATTTATAACAATAAGCTTATTT
>LE_HBa0150A22_T7_90283 (trimmed for quality and cloning vector)
AGCTTTTTTTACAATTCTAATGTCAACCCTTCAGAAACCGATCTACACGACTCTCTACGAGTTCGTCACAATCCAGTAGCTAGCTTTATTCTAAAAATCC
ACTCAAAATCCTAACTCTACATCCACAAACAAATATCTAATACTCCCTCCTGTACCAATTTTAAGTGTCTTACTTTCCTTTTTGGAATGTCTCTTCCTAT
ATTTCATTAGTTCTTCAATTGCAAAATTAAGACCCCGAGATTTAAAGGACTACACACATCTTTCTAAAACTATACTTTTTGAAGCTCAATTTTGAAATAC
TAAAAGGGAACTGAAATCAAATCAAATTTTCAAAATAAACCCCAATTTAAGAGAGAGAAAGTAAGTTACCATCCAATAAGGTAACCAAAAGCAATGAAGA
AAGTGATGATCCCACAATAAGGCCGATAGATAACCATAGAAGCATAACCCAAAAGCATAGGCACCAAAAAGTGAAGATTTGAACTGAATCCGAAAGACAA
GTATGAAAGAACACCACCCATCACGGCGGCGTAGAGATGTCTTCCGGCGGAAGAAGGAAC

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.942159
Expected error %
1.06013
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g12640.1 Score 105 E Value 2e-23 Identity 25.90% Align. Length 188bp (33.57%) Frame 1
68414.m01468 membrane bound O-acyl transferase (MBOAT) family protein low similarity to porcupine from [Xenopus laevis] GI:6714514, GI:6714520, GI:6714518, GI:6714516; contains Pfam profile PF03062: MBOAT family
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At1g63050.1 Score 98 E Value 3e-21 Identity 23.30% Align. Length 188bp (33.57%) Frame 1
68414.m07122 membrane bound O-acyl transferase (MBOAT) family protein low similarity to porcupine from [Xenopus laevis] GI:6714514, GI:6714520, GI:6714518, GI:6714516; contains Pfam profile PF03062: MBOAT family
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
gb|AAM91162.1| Score 105 E Value 7e-22 Identity 25.90% Align. Length 188bp (33.57%) Frame 1
unknown protein [Arabidopsis thaliana] gb|AAM13086.1| unknown protein [Arabidopsis thaliana]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
ref|NP_172724.2| Score 105 E Value 7e-22 Identity 25.90% Align. Length 188bp (33.57%) Frame 1
membrane bound O-acyl transferase (MBOAT) family protein [Arabidopsis thaliana]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0150A22
Clone
Clone name
hba-150a22
LE_HBa0150A22
P150A22
Clone type
BAC
Contamination
none
Estimated length
not yet estimated
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found