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SC Spread ID	Images			Distance from centromere (percentage of arm length)
555-02 1	Composite	Fluorescence	Phase	21.5%
555-06 1	Composite	Fluorescence	Phase	23.8%
555-08 1	Composite	Fluorescence	Phase	20.0%
555-11 1	Composite	Fluorescence	Phase	20.1%
555-13 1	Composite	Fluorescence	Phase	20.9%
555-15 1	Composite	Fluorescence	Phase	20.0%
555-16 1	Composite	Fluorescence	Phase	22.3%
555-22 1	Composite	Fluorescence	Phase	23.5%
555-23 1	Composite	Fluorescence	Phase	20.0%
555-26 1	Composite	Fluorescence	Phase	18.1%
555-28 1	Composite	Fluorescence	Phase	19.8%
555-32 1	Composite	Fluorescence	Phase	19.0%
555-33 1	Composite	Fluorescence	Phase	20.6%
Mean±sd				20.7%±1.7%

Given an estimated length of 20.1µm for chromosome 1Q and the mean percentage above, this BAC is approximately 4.2µm±0.3µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.

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