Clone hba-20f17

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Clone
Clone name
hba-20f17
LE_HBa0020F17
C04HBa0020F17
P020F17
Clone type
BAC
Contamination
none
Estimated length
123,163
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII
Ordering Information
BAC Clones
BAC clones can be ordered from the clone ordering page at TFGD
Physical mapping  
Fingerprint Contig Builds (FPC) 
not present in Tomato FPC (AGI 2005)
Marker matches - overgo what's this?None
Marker matches - BLASTNone
Marker matches - manual 
Marker
HBa20F17
Publication
unpublished
Submitted by
Lukas Mueller (Boyce Thompson Institute for Plant Research)
Comment
Manual associations of BACs with mapped BAC ends. Mapping by Yimin Xu, Cornell University
Physical map (from marker matches) 
View on physical map chromosome 4
FISH map 
View on FISH map chromosome 4
FISH images 
SC Spread IDImagesDistance from centromere
(percentage of arm length)
160-02 1CompositeFluorescencePhase39.3%
160-05 1CompositeFluorescencePhase40.8%
160-07 1CompositeFluorescencePhase40.9%
160-08 1CompositeFluorescencePhase40.5%
160-09 1CompositeFluorescencePhase40.2%
160-19 1CompositeFluorescencePhase43.2%
160-27 1CompositeFluorescencePhase39.9%
160-31 1CompositeFluorescencePhase40.7%
Mean±sd40.7%±1.2%

Given an estimated length of 13.2µm for chromosome 4Q and the mean percentage above, this BAC is approximately 5.4µm±0.2µm from the chromosome centromere.

1Experiment conducted in the lab of Stephen Stack at Colorado State University.
IL MappingNone
Sequencing  
This clone is being sequenced by the Chromosome 4 Sequencing Project. (View projects)
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Sequencing Project
Chromosome 4
HTGS phase
3 - finished
Sequencing Status
complete
Sequencing Organization
not recorded
Full Sequence
128,461 bp
C04HBa0020F17.1
CT990489.3 (GenBank) 		[View]
End Sequences
Warnings
none
FTP
Chromosome 4 Sequencing repository (FTP)
Chromosome Assembly
this clone is not part of a chromosome assembly
  
Restriction Fragments
HindIII in vitro (27)
1502, 1616, 1915, 2100, 2112, 2219, 2286, 2386, 2394, 2572, 2734, 2752, 2886, 3063, 3133, 3287, 3857, 4192, 4518, 6184, 6343, 6514, 8541, 8705, 9171, 10575, 15606
HindIII in silico (30)
1052, 1154, 1392, 1498, 1610, 1891, 2077, 2114, 2213, 2295, 2399, 2402, 2586, 2742, 2779, 2878, 3057, 3139, 3307, 3887, 4199, 4453, 5941, 6172, 6381, 8381, 8579, 9043, 10407, 15183
Fragment Lengths Match Score
0.93
Annotations  
Browse
LE_HBa0020F17 has no browsable sequence annotations
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End Sequences

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Map
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Chromosome / Linkage Group
  
IL Bin Mapping
Assigned to project
Mapped to chr
Mapped to bin
  
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