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Clone Read LE_HBa0020F17_T7_132742

Sequence Read 
Read Class
BAC ends
Read ID
LE_HBa0020F17_T7_132742
Primer
T7
ID from Seq. Facility
LE_HBa_020_F17-T7
Chromatogram
[download]
Sequence 
Version
1 (current)
Length (bp)
819 trimmed for vector and quality, 998 raw
Genbank Accession
DU051698
Sequence
>LE_HBa0020F17_T7_132742 (raw untrimmed)
CCTACTCGGTACCGGGGGGTCCTCTAGAGTCGACCTGCAGGCATGCAAGCTCTGTGGGGAGTAGGGGTTCAAAAACTCTATACTTTGATTTTGACAAATT
GGCAGAGGTTTGTTTTGTTCTAAATACCAACTTTCTTGAACATGTTTCTCTTGACATTATTCTTAATTTTGCTCTGTTTGCCATGTGAGACAGGTCACTG
CATTAGTCACTTCTAACCTGAACAAAATTATTGACGTCAATTACTACCCTGTTGAAACTGCAAAAAGGTCTAACTTACGACATAGGCCTATTGGACTTGG
AGTCCAGGGACTTGCAGACACATTCATTTTGCTTGGCATGGCATTTGATTCCCAGGAGGTAAGTTTTGTCTATCATCTTTTCTAGCTGTACATTTGTGCA
GATTGTTCTGATCTTATACTTTGTCTATGTATAGGCTCAGCAGCTAAACAAGGACATATTTGAGACAATATACTACCATGCATTAAAAGCTTCTTCTGAA
TTAGCTGCCAAGGAAGGCCCATACGAGACATATGCAGGAAGTCCTGTAAGCAAGGTATAAATCCTTTCATTCCTAGATTTTACAAATGGGTAATATGTTA
ATGGGATGTTTGATTTTCCAGTGCATATTGACGATCATCCATGATTTAGTCGAAATGGTTGTTATACTTCTAAGTGTCTAACATAAATAAAATAGCATCT
TCTTACTGGTTTGACTTTGAAGATATGATTTGCAGTTTAAAGAGAATTTGAAAATGTTGAGACACAAATTATACACACTACAGTAAAACATAAAATGACT
GTCAGATTTTATTTTGATTCACATTGTATTTCCCCTAAATACTTATTATGCTGCCCAGGGTATTCTCAACCAAGACATTTGGGGATTACCCCATCTAATA
AATCGGATTGGACTCCTTTCCTGAAATGATTGCAATAAAGGTGAAAAAAATTCCTTCCTGAACTCCAATGCCAACGCTTTCACCCGCCAATTCTTGAA
>LE_HBa0020F17_T7_132742 (trimmed for quality and cloning vector)
AGCTCTGTGGGGAGTAGGGGTTCAAAAACTCTATACTTTGATTTTGACAAATTGGCAGAGGTTTGTTTTGTTCTAAATACCAACTTTCTTGAACATGTTT
CTCTTGACATTATTCTTAATTTTGCTCTGTTTGCCATGTGAGACAGGTCACTGCATTAGTCACTTCTAACCTGAACAAAATTATTGACGTCAATTACTAC
CCTGTTGAAACTGCAAAAAGGTCTAACTTACGACATAGGCCTATTGGACTTGGAGTCCAGGGACTTGCAGACACATTCATTTTGCTTGGCATGGCATTTG
ATTCCCAGGAGGTAAGTTTTGTCTATCATCTTTTCTAGCTGTACATTTGTGCAGATTGTTCTGATCTTATACTTTGTCTATGTATAGGCTCAGCAGCTAA
ACAAGGACATATTTGAGACAATATACTACCATGCATTAAAAGCTTCTTCTGAATTAGCTGCCAAGGAAGGCCCATACGAGACATATGCAGGAAGTCCTGT
AAGCAAGGTATAAATCCTTTCATTCCTAGATTTTACAAATGGGTAATATGTTAATGGGATGTTTGATTTTCCAGTGCATATTGACGATCATCCATGATTT
AGTCGAAATGGTTGTTATACTTCTAAGTGTCTAACATAAATAAAATAGCATCTTCTTACTGGTTTGACTTTGAAGATATGATTTGCAGTTTAAAGAGAAT
TTGAAAATGTTGAGACACAAATTATACACACTACAGTAAAACATAAAATGACTGTCAGATTTTATTTTGATTCACATTGTATTTCCCCTAAATACTTATT
ATGCTGCCCAGGGTATTCT

Warnings:
  • Not screened for repetitive elements
  • Not screened for chimera
Quality Report
Processed by
SGN
Vector signature
5' read, flanking vector found
Quality trim threshold
12.5
Sequence entropy
0.955364
Expected error %
1.4376
Sequence Annotations 
Automatic Annotations
Arabidopsis proteins (TAIR10) (ath/ATH_pep)NCBI BLAST
At2g21790.1 Score 97 E Value 9e-41 Identity 78.50% Align. Length 497bp (60.68%) Frame 2
68415.m02590 ribonucleoside-diphosphate reductase small chain, putative / ribonucleotide reductase, putative similar to ribonucleotide reductase GI:4151068 from [Nicotiana tabacum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA71816.1| Score 107 E Value 8e-46 Identity 87.90% Align. Length 500bp (61.05%) Frame 2
ribonucleotide reductase [Nicotiana tabacum]
NCBI NR non-redundant protein sequence database (genbank/nr)NCBI BLAST
emb|CAA71815.1| Score 107 E Value 1e-46 Identity 89.40% Align. Length 500bp (61.05%) Frame 2
ribonucleotide reductase [Nicotiana tabacum]
Manual Annotations
No manual annotations found.
BAC 
Clone LE_HBa0020F17
Clone
Clone name
hba-20f17
LE_HBa0020F17
C04HBa0020F17
P020F17
Clone type
BAC
Contamination
none
Estimated length
123,163
Library
Name
Tomato HindIII BAC Library
Cloning host
E. coli
Organism
Solanum lycopersicum (formerly Lycopersicon esculentum)
Cloning vector
pBeloBAC11
Accession
Heinz 1706
Restriction enzyme
HindIII

Other Reads:
1 sequencing reads found